Journal article
Estrogen Receptors Are Found in Glia and at Extranuclear Neuronal Sites in the Dorsal Striatum of Female Rats: Evidence for Cholinergic But Not Dopaminergic Colocalization
Endocrinology (Philadelphia), Vol.153(11), pp.5373-5383
11/01/2012
DOI: 10.1210/en.2012-1458
PMCID: PMC3473205
PMID: 22919059
Abstract
Abstract Estrogens rapidly affect dopamine (DA) neurotransmission in the dorsal striatum (dSTR) and DA-related diseases, such as Parkinson's disease and schizophrenia. How estrogens influence DA function remains unclear, in part, because the ultrastructural localization of estrogen receptors (ER) in the dSTR is not known. Light microscopic studies of the dSTR have suggested the presence of ER. This experiment used electron microscopy to determine whether these ER are at extranuclear sites in the dSTR, providing evidence for a mechanism through which estrogen could rapidly affect DA transmission. The dSTR was labeled with antibodies for ERα, ERβ, and G protein-coupled ER 1 (GPER-1) to confirm whether these ER were present in this brain area. After this, the dSTR was dual labeled with antibodies for ERα or GPER-1 and tyrosine hydroxylase or vesicular acetylcholine transporter to determine whether ER are localized to dopaminergic and/or cholinergic processes, respectively. Ultrastructural analysis revealed immunoreactivity (IR) for ERα, ERβ, and GPER-1 exclusively at extranuclear sites throughout the dSTR. ERα-, ERβ-, and GPER-1-IR are mostly frequently observed in axons and glial profiles but are also localized to other neuronal profiles. Dual labeling revealed that ERα- and GPER-1-IR is not associated with DA axons and terminals but is sometimes associated with cholinergic neurons. Because these receptors are exclusively extranuclear in the dSTR, binding at these receptors likely affects neurotransmission via nongenomic mechanisms.
Details
- Title: Subtitle
- Estrogen Receptors Are Found in Glia and at Extranuclear Neuronal Sites in the Dorsal Striatum of Female Rats: Evidence for Cholinergic But Not Dopaminergic Colocalization
- Creators
- Anne Almey - Centre for Studies in Behavioral Neurobiology (A.A., W.G.B.), Department of Psychology, Concordia University, Montreal, Quebec, Canada H4B 1R6Edward J Filardo - and Department of Medicine (E.J.F.), Brown University, Providence, Rhode Island 02903Teresa A Milner - Department of Neurology and Neuroscience (T.A.M.), Weill Cornell Medical College, The Rockefeller University, New York, New York 10065, Harold and Margaret Milliken Hatch Laboratory of Neuroendocrinology (T.A.M.), The Rockefeller University, New York, New York 10065Wayne G Brake - Centre for Studies in Behavioral Neurobiology (A.A., W.G.B.), Department of Psychology, Concordia University, Montreal, Quebec, Canada H4B 1R6
- Resource Type
- Journal article
- Publication Details
- Endocrinology (Philadelphia), Vol.153(11), pp.5373-5383
- DOI
- 10.1210/en.2012-1458
- PMID
- 22919059
- PMCID
- PMC3473205
- ISSN
- 0013-7227
- eISSN
- 1945-7170
- Language
- English
- Date published
- 11/01/2012
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Surgery; Internal Medicine
- Record Identifier
- 9984051569202771
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