Journal article
Ethanol Inhibits Basic Fibroblast Growth Factor‐Mediated Proliferation of C6 Astrocytoma Cells
Journal of neurochemistry, Vol.67(4), pp.1448-1456
10/1996
DOI: 10.1046/j.1471-4159.1996.67041448.x
PMID: 8858926
Abstract
: Early ethanol exposure alters the proliferative activity of glial and neuronal precursors in the developing CNS. The present study tests the hypothesis that ethanol‐induced alterations in cell proliferation result from interference with growth factors. An in vitro model of astroglia (C6 astrocytoma cells) was used to study the effects of ethanol on proliferation mediated by basic fibroblast growth factor (bFGF). bFGF stimulated the proliferation of C6 cells. This bFGF‐enhanced proliferation was evident by increases in total cell number, DNA synthesis (as measured by [3H]thymidine incorporation), and the number of cells that took up bromodeoxyuridine. A synthetic peptide that specifically blocked the binding of bFGF to its high‐affinity receptor completely abolished the proliferation‐promoting effect of bFGF. The action of another mitogen for C6 cells, insulin‐like growth factor‐1, was not affected by this peptide. Therefore, the bFGF‐stimulated proliferation was mediated through a specific bFGF receptor. Ethanol inhibited bFGF‐mediated proliferation in a concentration‐dependent manner. Ethanol concentrations of 100 and 200 mg/dl partially inhibited bFGF‐mediated proliferation (by 58 and 74%, respectively), whereas concentrations of ≥400 mg/dl completely abolished the growth‐stimulating effect of bFGF. Our data show that ethanol alters proliferative activity of C6 cells by disrupting the action of bFGF. The target of ethanol neurotoxicity is a receptor‐mediated activity. bFGF can affect cell proliferation by a non‐receptor‐mediated intracellular pathway, but ethanol does not have an impact on this pathway.
Details
- Title: Subtitle
- Ethanol Inhibits Basic Fibroblast Growth Factor‐Mediated Proliferation of C6 Astrocytoma Cells
- Creators
- Jia Luo - University of IowaMichael W Miller - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of neurochemistry, Vol.67(4), pp.1448-1456
- Publisher
- Blackwell Science Ltd
- DOI
- 10.1046/j.1471-4159.1996.67041448.x
- PMID
- 8858926
- ISSN
- 0022-3042
- eISSN
- 1471-4159
- Language
- English
- Date published
- 10/1996
- Academic Unit
- Pathology
- Record Identifier
- 9984186539902771
Metrics
7 Record Views