Ethnicity and Smoking-Associated DNA Methylation Changes at HIV Co-Receptor GPR15

Meeshanthini V Dogan; Jinhua Xiang; Steven R. H Beach; Carolyn Cutrona; Frederick X Gibbons; Ronald L Simons; Gene H Brody; Jack T Stapleton; Robert A Philibert

doi:10.3389/fpsyt.2015.00132

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Ethnicity and Smoking-Associated DNA Methylation Changes at HIV Co-Receptor GPR15

Journal article

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Ethnicity and Smoking-Associated DNA Methylation Changes at HIV Co-Receptor GPR15

Meeshanthini V Dogan, Jinhua Xiang, Steven R. H Beach, Carolyn Cutrona, Frederick X Gibbons, Ronald L Simons, Gene H Brody, Jack T Stapleton and Robert A Philibert

Frontiers in psychiatry, Vol.6, pp.132-132

2015

DOI: 10.3389/fpsyt.2015.00132

PMCID: PMC4585036

PMID: 26441693

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Abstract

Smoking is associated with poorer health outcomes for both African and European Americans. In order to better understand whether ethnic-specific genetic variation may underlie some of these differences, we compared the smoking-associated genome-wide methylation signatures of African Americans with those of European Americans, and followed up this analysis with a focused examination of the most ethnically divergent locus, cg19859270, at the GPR15 gene. We examined the association of methylation at this locus to the rs2230344 SNP and GPR15 gene and protein expression. Consistent with prior analyses, AHRR residue cg05575921 was the most differentially methylated residue in both African Americans and European Americans. However, the second most differentially methylated locus in African Americans, cg19859270, was only modestly differentially methylated in European Americans. Interrogation of the methylation status of this CpG residue found in GPR15, a chemokine receptor involved in HIV pathogenesis, showed a significant interaction of ethnicity with smoking as well as a marginal effect of genotype at rs2230344, a neighboring non-synonymous SNP, but only among African Americans. Gene and protein expression analyses showed that demethylation at cg19859270 was associated with an increase in both mRNA and protein levels. Since GPR15 is involved in the early stages of viral replication for some HIV-1 and HIV-2 isolates, and the prevalence of HIV is increased in African Americans and smokers, these data support a possible role for GPR15 in the ethnically dependent differential prevalence of HIV.

GPR15

HIV

ethnicity

smoking

African Americans

AHRR

Psychiatry

epigenetics

European Americans

Details

Title: Subtitle: Ethnicity and Smoking-Associated DNA Methylation Changes at HIV Co-Receptor GPR15
Creators: Meeshanthini V Dogan - Department of Biomedical Engineering, University of Iowa
Jinhua Xiang - Department of Internal Medicine, University of Iowa
Steven R. H Beach - Center for Family Research, University of Georgia
Carolyn Cutrona - Department of Psychology, Iowa State University
Frederick X Gibbons - Department of Psychology, University of Connecticut
Ronald L Simons - Center for Family Research, University of Georgia
Gene H Brody - Center for Family Research, University of Georgia
Jack T Stapleton - Department of Internal Medicine, University of Iowa
Robert A Philibert - Department of Biomedical Engineering, University of Iowa
Resource Type: Journal article
Publication Details: Frontiers in psychiatry, Vol.6, pp.132-132
DOI: 10.3389/fpsyt.2015.00132
PMID: 26441693
PMCID: PMC4585036
NLM abbreviation: Front Psychiatry
ISSN: 1664-0640
eISSN: 1664-0640
Publisher: Frontiers Media S.A
Language: English
Date published: 2015
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Psychiatry; Microbiology and Immunology; Infectious Diseases; Iowa Neuroscience Institute; Internal Medicine
Record Identifier: 9984070577802771

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