Evaluating the relationship between acidogenicity and acid tolerance for oral streptococci from children with or without a history of caries

Jeffrey A. Banas; Erika Takanami; Ryan M. Hemsley; Alissa Villhauer; Min Zhu; Fang Qian; Amber Marolf; David R. Drake

doi:10.1080/20002297.2019.1688449

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Evaluating the relationship between acidogenicity and acid tolerance for oral streptococci from children with or without a history of caries

Journal article

Open access

Peer reviewed

Evaluating the relationship between acidogenicity and acid tolerance for oral streptococci from children with or without a history of caries

Jeffrey A. Banas, Erika Takanami, Ryan M. Hemsley, Alissa Villhauer, Min Zhu, Fang Qian, Amber Marolf and David R. Drake

Journal of oral microbiology, Vol.12(1), pp.1688449-1688449

01/01/2020

DOI: 10.1080/20002297.2019.1688449

PMCID: PMC6844425

PMID: 31893016

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Abstract

Background: Dental caries etiology is attributed to a dysbiotic imbalance within the plaque microbiome leading to a dominance of strong acidogens. Some studies that investigate the link between acidogens and caries quantify the recovery of acid tolerant strains on acid agar as a measure of acidogenic potential. This methodology assumes that acidogenic potential and acid tolerance are directly related. Aim: The validity of that assumption was investigated by statistically evaluating that relationship using streptococci recovered from children with or without a history of dental caries. Methods: Thirty streptococcal isolates were isolated from each of 13 subjects. Acidogenicity was quantified by measuring the terminal pH after overnight growth in Brain Heart Infusion (BHI) and Chemically Defined Medium (CDM). Acid tolerance was quantified by measuring the lowest pH acid agar displaying growth. Results: A significant difference in acidogenicity in CDM between levels of acid tolerance was found, but no significant difference in acidogenicity in BHI was noted. Moreover, there were no significant interactions between acid tolerance and caries history on acidogenicity measures in either medium. Conclusion: An ability to grow on acid agar below pH 5.0 is best aligned with strong acidogenicity and best able to distinguish between subjects with differing caries histories.

Life Sciences & Biomedicine

Microbiology

Science & Technology

Details

Title: Subtitle: Evaluating the relationship between acidogenicity and acid tolerance for oral streptococci from children with or without a history of caries
Creators: Jeffrey A. Banas - University of Iowa
Erika Takanami - University of Iowa
Ryan M. Hemsley - University of Iowa
Alissa Villhauer - University of Iowa
Min Zhu - University of Iowa
Fang Qian - University of Iowa
Amber Marolf - University of Iowa
David R. Drake - University of Iowa
Resource Type: Journal article
Publication Details: Journal of oral microbiology, Vol.12(1), pp.1688449-1688449
DOI: 10.1080/20002297.2019.1688449
PMID: 31893016
PMCID: PMC6844425
NLM abbreviation: J Oral Microbiol
ISSN: 2000-2297
eISSN: 2000-2297
Publisher: Taylor & Francis
Number of pages: 6
Grant note: DE026716 / National Institute of Dental & Craniofacial Research; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR)
Language: English
Date published: 01/01/2020
Academic Unit: Preventive and Community Dentistry; Endodontics; Pediatric Dentistry; Dental Research
Record Identifier: 9984367736202771

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