Journal article
Evaluation of Early Biomarkers of Atherosclerosis Associated with Polychlorinated Biphenyl Exposure: An in Vitro and in Vivo Study
Environmental health perspectives, Vol.130(3), pp.37011-37011
03/2022
DOI: 10.1289/EHP9833
PMCID: PMC8963524
PMID: 35349355
Abstract
Miscellaneous cardiovascular risk factors have been defined, but the contribution of environmental pollutants exposure on cardiovascular disease (CVD) remains underappreciated.
We investigated the potential impact of typical environmental pollutant exposure on atherogenesis and its underlying mechanisms.
We used human umbilical vein endothelial cells (HUVECs) and apolipoprotein E knockout (
) mice to investigate how 2,3,5-trichloro-6-phenyl-[1,4]-benzoquinone (PCB29-pQ, a toxic polychlorinated biphenyl metabolite) affects atherogenesis and identified early biomarkers of CVD associated with PCB29-pQ exposures. Then, we used long noncoding RNAs (lncRNAs)
-overexpressing
mice and apolipoprotein E/caveolin 1 double-knockout (
) mice to address the role of these early biomarkers in PCB29-pQ-induced atherogenesis. Plasma samples from patients with coronary heart disease (CHD) were also used to confirm our findings.
Our data indicate that lncRNA
bound to
via argonaute 2 in PCB29-pQ-challenged HUVECs. Our mRNA sequencing assay identified transforming growth
(
) as a possible target gene of
;
sponged
and inhibited the binding of
to
. The effect of PCB29-pQ-induced endothelial injury, vascular inflammation, development of plaques, and atherogenesis in
mice was greater with
-mediated
inhibition, whereas
-overexpressing
mice and
mice showed the opposite effect. Consistently, plasma levels of
and
were found to be significantly associated individuals diagnosed with CHD.
These findings demonstrated that a mechanism-based, integrated-omics approach enabled the identification of potentially clinically relevant diagnostic indicators and therapeutic targets of CHD mediated by environmental contaminants using
and
models of HUVECs and
and
mice. https://doi.org/10.1289/EHP9833.
Details
- Title: Subtitle
- Evaluation of Early Biomarkers of Atherosclerosis Associated with Polychlorinated Biphenyl Exposure: An in Vitro and in Vivo Study
- Creators
- Bingwei Yang - Southwest UniversityZhishuai Ye - Capital Medical UniversityYawen Wang - Southwest UniversityHongzhou Guo - Capital Medical UniversityHans-Joachim Lehmler - Department of Occupational and Environmental Health, University of Iowa, Iowa City, Iowa, USARongchong Huang - Capital Medical UniversityErqun Song - Southwest UniversityYang Song - Research Center for Eco-Environmental Sciences
- Resource Type
- Journal article
- Publication Details
- Environmental health perspectives, Vol.130(3), pp.37011-37011
- DOI
- 10.1289/EHP9833
- PMID
- 35349355
- PMCID
- PMC8963524
- NLM abbreviation
- Environ Health Perspect
- eISSN
- 1552-9924
- Language
- English
- Date published
- 03/2022
- Academic Unit
- Occupational and Environmental Health; Iowa Neuroscience Institute; Iowa Superfund Research Program
- Record Identifier
- 9984230415502771
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