Evaluation of Etest and disk diffusion methods compared with broth microdilution antifungal susceptibility testing of clinical isolates of Candida spp. against posaconazole

Daniel J Diekema; Shawn A Messer; Richard J Hollis; Linda B Boyken; Shailesh Tendolkar; Jennifer Kroeger; Michael A Pfaller

doi:10.1128/JCM.02087-06

Back

Evaluation of Etest and disk diffusion methods compared with broth microdilution antifungal susceptibility testing of clinical isolates of Candida spp. against posaconazole

Journal article

Open access

Peer reviewed

Evaluation of Etest and disk diffusion methods compared with broth microdilution antifungal susceptibility testing of clinical isolates of Candida spp. against posaconazole

Daniel J Diekema, Shawn A Messer, Richard J Hollis, Linda B Boyken, Shailesh Tendolkar, Jennifer Kroeger and Michael A Pfaller

Journal of clinical microbiology, Vol.45(6), pp.1974-1977

06/2007

DOI: 10.1128/JCM.02087-06

PMCID: PMC1933029

PMID: 17301284

Files and links (1)

url

https://doi.org/10.1128/JCM.02087-06View

Published (Version of record) Open Access

Abstract

We performed Etest, disk diffusion, and broth microdilution susceptibility testing of 2,171 clinical isolates of Candida spp. against posaconazole. By using provisional breakpoints for comparison purposes only, the categorical agreement between the agar-based methods and broth microdilution results ranged from 93 to 98%, with <1% very major errors. The essential agreement (within 2 well dilutions) between the Etest and broth microdilution methods was 94%. These agar-based methods hold promise as simple and reliable methods for determination of the posaconzole susceptibilities of Candida spp.

Antifungal Agents - pharmacology

Disk Diffusion Antimicrobial Tests - standards

Disk Diffusion Antimicrobial Tests - methods

Humans

Agar

Candidiasis - microbiology

Candida - isolation & purification

Triazoles - pharmacology

Candida - classification

Microbial Sensitivity Tests - standards

Culture Media

Candida - drug effects

Microbial Sensitivity Tests - methods

Details

Title: Subtitle: Evaluation of Etest and disk diffusion methods compared with broth microdilution antifungal susceptibility testing of clinical isolates of Candida spp. against posaconazole
Creators: Daniel J Diekema - Department of Pathology, Roy J. and Lucille A. Carver College of Medicine and College of Public Health, University of Iowa, Iowa City, IA 52242, USA. daniel-diekema@uiowa.edu
Shawn A Messer
Richard J Hollis
Linda B Boyken
Shailesh Tendolkar
Jennifer Kroeger
Michael A Pfaller
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.45(6), pp.1974-1977
DOI: 10.1128/JCM.02087-06
PMID: 17301284
PMCID: PMC1933029
NLM abbreviation: J Clin Microbiol
ISSN: 0095-1137
eISSN: 1098-660X
Publisher: United States
Language: English
Date published: 06/2007
Academic Unit: Infectious Diseases; Epidemiology; Pathology; Internal Medicine
Record Identifier: 9983986258102771

Metrics

29 Record Views

23 Times Cited - Web of Science