Evaluation of Etest method for determining fluconazole and voriconazole MICs for 279 clinical isolates of Candida species infrequently isolated from blood

M J Maxwell; S A Messer; R J Hollis; L Boyken; S Tendolkar; D J Diekema; M A Pfaller

doi:10.1128/JCM.41.3.1087-1090.2003

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Evaluation of Etest method for determining fluconazole and voriconazole MICs for 279 clinical isolates of Candida species infrequently isolated from blood

Journal article

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Evaluation of Etest method for determining fluconazole and voriconazole MICs for 279 clinical isolates of Candida species infrequently isolated from blood

M J Maxwell, S A Messer, R J Hollis, L Boyken, S Tendolkar, D J Diekema and M A Pfaller

Journal of clinical microbiology, Vol.41(3), pp.1087-1090

03/2003

DOI: 10.1128/JCM.41.3.1087-1090.2003

PMCID: PMC150324

PMID: 12624034

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Abstract

The performance of Etest in fluconazole and voriconazole testing of 279 isolates of uncommon Candida spp. was assessed in comparison with the National Committee for Clinical Laboratory Standards (NCCLS)-approved standard broth microdilution (BMD) method. The NCCLS method employed RPMI 1640 broth medium, and MICs were read after incubation for 48 h at 35 degrees C. Etest MICs were determined with RPMI agar containing 2% glucose and were read after incubation for 48 h at 35 degrees C. The isolates include Candida krusei, C. lusitaniae, C. guilliermondii, C. kefyr, C. rugosa, C. lipolytica, C. pelliculosa, C. dubliniensis, C. famata, C. zeylanoides, C. inconspicua, and C. norvegensis. Overall agreement between Etest and BMD MICs was 96% for fluconazole and 95% for voriconazole. Where a discrepancy was observed between Etest and the reference method, the Etest tended to give lower values with both fluconazole and voriconazole. The Etest method using RPMI agar appears to be a useful method for determining fluconazole and voriconazole susceptibilities of uncommon species of Candida.

Antifungal Agents - pharmacology

Triazoles - pharmacology

Fluconazole - pharmacology

Humans

Voriconazole

Blood - microbiology

Candida - drug effects

Pyrimidines - pharmacology

Microbial Sensitivity Tests - methods

Details

Title: Subtitle: Evaluation of Etest method for determining fluconazole and voriconazole MICs for 279 clinical isolates of Candida species infrequently isolated from blood
Creators: M J Maxwell - Departments of Pathology. Medicine. Epidemiology, University of Iowa College of Medicine and College of Public Health, Iowa City, Iowa 52242, USA
S A Messer
R J Hollis
L Boyken
S Tendolkar
D J Diekema
M A Pfaller
Contributors: International Fungal Surveillance Participant Group (Author)
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.41(3), pp.1087-1090
DOI: 10.1128/JCM.41.3.1087-1090.2003
PMID: 12624034
PMCID: PMC150324
NLM abbreviation: J Clin Microbiol
ISSN: 0095-1137
eISSN: 1098-660X
Publisher: United States
Language: English
Date published: 03/2003
Academic Unit: Infectious Diseases; Epidemiology; Pathology; Internal Medicine
Record Identifier: 9983986257302771

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