Journal article
Evaluation of Methods for Sequence Analysis of Highly Repetitive DNA Templates
Journal of biomolecular techniques, Vol.17(2), pp.138-144
04/01/2006
PMCID: PMC2291771
PMID: 16741241
Abstract
The DNA Sequencing Research Group (DSRG) of the ABRF conducted a study to assess the ability of DNA sequencing core facilities to successfully sequence a set of well-defined templates containing difficult repeats. The aim of this study was to determine whether repetitive templates could be sequenced accurately by using equipment and chemistries currently utilized in participating sequencing laboratories. The effects of primer and template concentrations, sequencing chemistries, additives, and instrument formats on the ability to successfully sequence repeat elements were examined. The first part of this study was an analysis of the results of 361 chromatograms from participants representing 40 different laboratories who attempted to sequence a panel of difficult-to-sequence templates using their best in-house protocols. The second part of this study was a smaller multi-laboratory evaluation of a single robust protocol with the same panel of templates. This study provides a measure of the potential success of different approaches to sequencing across homopolymer tracts and repetitive elements.
Details
- Title: Subtitle
- Evaluation of Methods for Sequence Analysis of Highly Repetitive DNA Templates
- Creators
- John W. HawesKevin L. KnudtsonHelaman EscobarGeorge S. GrillsTimothy C. HunterEmily Jackson-MachelskiHeather LinDavid S. NeedlemanRashmi PershadGlenis J. Wiebe
- Resource Type
- Journal article
- Publication Details
- Journal of biomolecular techniques, Vol.17(2), pp.138-144
- Publisher
- The Association of Biomolecular Resource Facilities
- PMID
- 16741241
- PMCID
- PMC2291771
- ISSN
- 1524-0215
- eISSN
- 1943-4731
- Language
- English
- Date published
- 04/01/2006
- Academic Unit
- Iowa Institute of Human Genetics
- Record Identifier
- 9984627217202771
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