Evaluation of Two Methods for Determination of CD64 as a Diagnostic Marker of Infection in Critically Ill Adults

Thiago Zinsly Sampaio Camargo; Alexandre R Marra; Nydia Strachman Bacal; Eduardo Casaroto; Lilian Moreira Pinto; Jacyr Pasternak; Elivane da Silva Victor; Oscar Fernando Pavão Dos Santos; Michael B Edmond

doi:10.1155/2016/6593232

Back

Evaluation of Two Methods for Determination of CD64 as a Diagnostic Marker of Infection in Critically Ill Adults

Journal article

Open access

Peer reviewed

Evaluation of Two Methods for Determination of CD64 as a Diagnostic Marker of Infection in Critically Ill Adults

Thiago Zinsly Sampaio Camargo, Alexandre R Marra, Nydia Strachman Bacal, Eduardo Casaroto, Lilian Moreira Pinto, Jacyr Pasternak, Elivane da Silva Victor, Oscar Fernando Pavão Dos Santos and Michael B Edmond

BioMed research international, Vol.2016, 6593232

2016

DOI: 10.1155/2016/6593232

PMCID: PMC5206427

PMID: 28097142

Files and links (1)

url

https://doi.org/10.1155/2016/6593232View

Published (Version of record) Open Access

Abstract

. Diagnostic markers of infection have had little innovation over the last few decades. CD64, a marker expressed on the surface of neutrophils, may have utility for this purpose. . This study was conducted in an adult intensive care unit (ICU) in São Paulo, Brazil, with 89 patients. We evaluated CD64 in patients with documented or clinically diagnosed infection (infection group) and controls (patients without any evidence of infection) by two different methodologies: method #1, an in house assay, and method #2, the commercial kit Leuko64 (Trillium Diagnostics). . CD64 displayed good discriminating power with a 91.2% sensitivity (95% CI 90.7-91.6%) for detecting infection. The commercial kit (Leuko64) demonstrated higher specificity (87.3%) compared with method #1 as well as better accuracy (88.8%). . CD64 seems to be a promising marker of infection in the intensive care setting, with Leuko64 showing a slight advantage.

Receptors, IgG - blood

Receptors, IgG - isolation & purification

Humans

Middle Aged

Critical Illness

Infection - blood

Male

Biomarkers - blood

Infection - pathology

Adult

Female

Aged

Neutrophils - pathology

Details

Title: Subtitle: Evaluation of Two Methods for Determination of CD64 as a Diagnostic Marker of Infection in Critically Ill Adults
Creators: Thiago Zinsly Sampaio Camargo - Intensive Care Unit, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil
Alexandre R Marra - Instituto Israelita de Ensino e Pesquisa Albert Einstein, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil
Nydia Strachman Bacal - Clinical Laboratory, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil
Eduardo Casaroto - Intensive Care Unit, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil
Lilian Moreira Pinto - Intensive Care Unit, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil
Jacyr Pasternak - Clinical Laboratory, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil
Elivane da Silva Victor - Instituto Israelita de Ensino e Pesquisa Albert Einstein, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil
Oscar Fernando Pavão Dos Santos - Division of Medical Practice, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil
Michael B Edmond - Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA, USA
Resource Type: Journal article
Publication Details: BioMed research international, Vol.2016, 6593232
DOI: 10.1155/2016/6593232
PMID: 28097142
PMCID: PMC5206427
NLM abbreviation: Biomed Res Int
ISSN: 2314-6133
eISSN: 2314-6141
Publisher: United States
Language: English
Date published: 2016
Academic Unit: Internal Medicine
Record Identifier: 9983905646302771

Metrics

43 Record Views

2 Times Cited - Web of Science