Journal article
Evaluation of a method for measuring the sustainable rate of sebaceous wax ester secretion
Archives of dermatological research (Print), Vol.279(4), pp.266-269
1987
DOI: 10.1007/BF00417326
PMID: 3674959
Abstract
Sebaceous was ester secretion rates were measured in six subjects on six occasions each, using absorption into bentonite clay and analysis of the collected lipid by quantitative thin-layer chromatography. On each occasion eight samples were collected, four from the left and four from the right side of the forehead in four successive intervals. The first two intervals, which totaled 14 h, were intended to deplete the follicular reservoir of sebum so that a constant rate of absorption could be obtained during the third and fourth intervals, which were 3 h each. Thin-layer analysis of each sample was done in triplicate. The data were examined using analysis of variance techniques to determine the reproducibility of the measurement method and to identify possible sources of variability. The intraclass correlation coefficient (r1) for all 432 post-depletion determinations was 0.80. The reproducibility was considerably better for three of the subjects (r1=0.93) than for the other three (r1=0.75). Variability within the latter three subjects did not seem to be attributable to lack of reproducibility in the thinlayer analysis. Real biological variability also seems unlikely considering the holocrine mechanisms of sebum secretion. Therefore, the variability probably arises from non-representative collection of sebum into the bentonite absorbent. © 1987 Springer-Verlag.
Details
- Title: Subtitle
- Evaluation of a method for measuring the sustainable rate of sebaceous wax ester secretion
- Creators
- D. W Collison - University of IowaT. L Burns - University of IowaM. E Stewart - Univ. Iowa coll. medicine, dep. dermatology, Iowa City IA 52242, United StatesD. T Downing - University of IowaJ. S Strauss - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Archives of dermatological research (Print), Vol.279(4), pp.266-269
- Publisher
- Springer
- DOI
- 10.1007/BF00417326
- PMID
- 3674959
- ISSN
- 0340-3696
- eISSN
- 1432-069X
- Language
- English
- Date published
- 1987
- Academic Unit
- Epidemiology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984363611302771
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