Journal article
Evaluation of a novel biocompatible magnetic nanomedicine based on beta-cyclodextrin, loaded doxorubicin-curcumin for overcoming chemoresistance in breast cancer
Artificial cells, nanomedicine, and biotechnology, Vol.46(2), pp.207-216
01/01/2018
DOI: 10.1080/21691401.2018.1453829
PMID: 29688063
Abstract
Codelivery of chemo-sensitizers with chemotherapeutics using combo nanomedicine is a promising platform for overcoming chemoresistance in breast cancer. However, tumor accumulation of nano-carriers based on enhanced permeability and retention (EPR) effect is confounded by heterogeneity in tumor microenvironment. Adsorption of protein corona on surface of nanoparticle boost up clearance by reticulo-endothelial system. In this study, a surface functionalized magnetic nanocomposite (NC) for codelivery of doxorubicin (DOX) and curcumin (CUR) is developed. NCs were coated with hydroxyapatite and were also cross linked with beta-cyclodextrin. NCs efficiently encapsulated DOX and CUR. Release of CUR and DOX were in a sustained pH-depended pattern. beta-cyclodextrin functionalization reduced protein corona according sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. As shown by flowcytometric and confocal microscopy analyses, NCs internalized efficiently by human breast carcinoma cells MCF-7 and adriamycin resistant MCF-7 (MCF-7/adr) cells. 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) test demonstrated superior cytotoxicity of DOX-CUR loaded NCs. Anti-tumor efficacy analyses confirmed reduction in relative tumor volume size (RTV%) compared to control group. Western blot analyses demonstrated marginal CUR mediated P-glycoprotein (P-gp) down regulation. DOX-CUR loaded NCs efficiently accumulated into the tumor via external magnet guidance. Nevertheless, the increased tumor accumulation did not correlate with pharmacologic responses such as RTV% and significant superiority over free DOX was not observed.
Details
- Title: Subtitle
- Evaluation of a novel biocompatible magnetic nanomedicine based on beta-cyclodextrin, loaded doxorubicin-curcumin for overcoming chemoresistance in breast cancer
- Creators
- Roghayeh Rastegar - Tehran University of Medical SciencesHamid Akbari Javar - Tehran University of Medical SciencesMehdi Khoobi - Tehran University of Medical SciencesPoua Dehghan Kelishadi - Tehran University of Medical SciencesGholam Hossein Yousefi - Shiraz Univ Med Sci, Dept Pharmaceut, Shiraz, IranMahmoud Doosti - Tehran University of Medical SciencesMohammad Hossien Ghahremani - Tehran University of Medical SciencesAhmad Shariftabrizi - University of Pittsburgh Medical CenterFatemeh Imanparast - Department of Medical Biochemistry, Irak University of Medical Sciences, Irak, Iran;Elham Gholibeglu - University of ZanjanMahdi Gholami - Tehran University of Medical Sciences
- Resource Type
- Journal article
- Publication Details
- Artificial cells, nanomedicine, and biotechnology, Vol.46(2), pp.207-216
- Publisher
- Taylor & Francis
- DOI
- 10.1080/21691401.2018.1453829
- PMID
- 29688063
- ISSN
- 2169-1401
- eISSN
- 2169-141X
- Number of pages
- 10
- Grant note
- 94-02-33-27729 / Tehran University of Medical Sciences (TUMS); Tehran University of Medical Sciences
- Language
- English
- Date published
- 01/01/2018
- Academic Unit
- Radiology
- Record Identifier
- 9984318784902771
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