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Evaluation of a rapid immunochromatographic test for detection of distinct variants of Klebsiella pneumoniae carbapenemase (KPC) in Enterobacteriaceae
Journal article   Open access   Peer reviewed

Evaluation of a rapid immunochromatographic test for detection of distinct variants of Klebsiella pneumoniae carbapenemase (KPC) in Enterobacteriaceae

Ana C Ramos, Ana C Gales, Jussimara Monteiro, Suzane Silbert, Thomás Chagas-Neto, Antonia M.O Machado and Cecilia G Carvalhaes
Journal of microbiological methods, Vol.142, pp.1-3
11/2017
DOI: 10.1016/j.mimet.2017.08.016
PMID: 28844719
url
https://doi.org/10.1016/j.mimet.2017.08.016View
Published (Version of record) Open Access

Abstract

The rapid detection of KPC-producing Enterobacteriaceae by microbiology laboratories has been required for infectious control programs. Herein we evaluated the performance of a novel immunochromatographic test for detecting KPC-2-, KPC-3-, KPC-4-, KPC-6-, KPC-7-, KPC-8-, and KPC-11-producing isolates and the influence of different growth media on the test performance. •Rapid detection of KPC-producing isolates is still a demanding issue on clinical practice.•A rapid immunochromatographic test was evaluated to detect different variants of KPC.•KPC-6, KPC-7, KPC-8 and KPC-11 variants were evaluated for the first time.•An enhancing on bacterial inoculum was proposed to detect all KPC variants tested.•No influence of commonly bacterial growth medium used on routine microbiology was observed.
Chromogenic agar Culture medium KPC variants KPC-K-SeT MacConkey Mueller-Hinton

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