Evaluation of an Immunochromatographic Assay for Rapid Detection of Penicillin-Binding Protein 2a in Human and Animal Staphylococcus intermedius Group, Staphylococcus lugdunensis, and Staphylococcus schleiferi Clinical Isolates

A R Arnold; C-A D Burnham; B A Ford; S D Lawhon; S K McAllister; D Lonsway; V Albrecht; R C Jerris; J K Rasheed; B Limbago; E M Burd; L F Westblade

doi:10.1128/JCM.02869-15

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Evaluation of an Immunochromatographic Assay for Rapid Detection of Penicillin-Binding Protein 2a in Human and Animal Staphylococcus intermedius Group, Staphylococcus lugdunensis, and Staphylococcus schleiferi Clinical Isolates

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Evaluation of an Immunochromatographic Assay for Rapid Detection of Penicillin-Binding Protein 2a in Human and Animal Staphylococcus intermedius Group, Staphylococcus lugdunensis, and Staphylococcus schleiferi Clinical Isolates

A R Arnold, C-A D Burnham, B A Ford, S D Lawhon, S K McAllister, D Lonsway, V Albrecht, R C Jerris, J K Rasheed, B Limbago, …

Journal of clinical microbiology, Vol.54(3), pp.745-748

03/2016

DOI: 10.1128/JCM.02869-15

PMCID: PMC4767977

PMID: 26677248

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Abstract

The performance of a rapid penicillin-binding protein 2a (PBP2a) detection assay, the Alere PBP2a culture colony test, was evaluated for identification of PBP2a-mediated beta-lactam resistance in human and animal clinical isolates of Staphylococcus intermedius group, Staphylococcus lugdunensis, and Staphylococcus schleiferi. The assay was sensitive and specific, with all PBP2a-negative and PBP2a-positive strains testing negative and positive, respectively.

Reproducibility of Results

Staphylococcus intermedius - isolation & purification

Staphylococcus lugdunensis - metabolism

Humans

Immunochromatography - methods

Penicillin-Binding Proteins - metabolism

Peptide Synthases - metabolism

Animals

Staphylococcus intermedius - metabolism

Sensitivity and Specificity

Staphylococcal Infections - microbiology

Staphylococcus lugdunensis - isolation & purification

Immunochromatography - standards

Details

Title: Subtitle: Evaluation of an Immunochromatographic Assay for Rapid Detection of Penicillin-Binding Protein 2a in Human and Animal Staphylococcus intermedius Group, Staphylococcus lugdunensis, and Staphylococcus schleiferi Clinical Isolates
Creators: A R Arnold - Emory University School of Medicine, Atlanta, Georgia, USA
C-A D Burnham - Washington University in St. Louis School of Medicine, St. Louis, Missouri, USA
B A Ford - University of Iowa Hospitals and Clinics, Iowa City, Iowa, USA
S D Lawhon - College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA
S K McAllister - Centers for Disease Control and Prevention, Atlanta, Georgia, USA
D Lonsway - Centers for Disease Control and Prevention, Atlanta, Georgia, USA
V Albrecht - Centers for Disease Control and Prevention, Atlanta, Georgia, USA
R C Jerris - Emory University School of Medicine, Atlanta, Georgia, USA Children's Healthcare of Atlanta, Atlanta, Georgia, USA
J K Rasheed - Centers for Disease Control and Prevention, Atlanta, Georgia, USA
B Limbago - Centers for Disease Control and Prevention, Atlanta, Georgia, USA
E M Burd - Emory University School of Medicine, Atlanta, Georgia, USA Emory Antibiotic Resistance Center, Atlanta, Georgia, USA eburd@emory.edu law9067@med.cornell.edu
L F Westblade - Weill Cornell Medical College, New York, New York, USA eburd@emory.edu law9067@med.cornell.edu
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.54(3), pp.745-748
Publisher: United States
DOI: 10.1128/JCM.02869-15
PMID: 26677248
PMCID: PMC4767977
ISSN: 0095-1137
eISSN: 1098-660X
Language: English
Date published: 03/2016
Academic Unit: Pathology
Record Identifier: 9984046811502771

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