Journal article
Evaluation of different therapeutic Carnoy's formulations on hard human tissues: A Raman microspectroscopy, microhardness, and scanning electron microscopy study
Journal of cranio-maxillo-facial surgery, Vol.46(5), pp.749-758
05/01/2018
DOI: 10.1016/j.jcms.2018.02.006
PMID: 29655532
Abstract
To evaluate different therapeutic Carnoy's solution formulations on hard human tissues.
An in vitro study was performed with human teeth (n = 36) and bone fragments (n = 18), randomly divided into two experimental groups (Group I = Carnoy solution with chloroform; Group II = Carnoy solution without chloroform) and a control group (saline solution). The groups were subdivided into pre-conditioning, post-conditioning, and post-conditioning with saline washing. Raman microspectroscopy, Knoop microhardness test, and scanning electron microscopy with energy dispersive X-ray spectroscopy were used.
There was demineralization of dental structures regarding mineral/matrix and carbonate/phosphate ratios (GI versus GII, p < 0.05). The presence of chloroform resulted in a statistically significant reduction of the teeth surface microhardness (p = 0.036), but not exceeding 0.01 μm. Both GI and GII showed significant structural changes by using scanning electron microscopy with energy dispersive X-ray spectroscopy.
Carnoy's solution altered the organic and inorganic matrix of the human calcified specimens analyzed in vitro, and its effect was more pronounced when chloroform was present.
Details
- Title: Subtitle
- Evaluation of different therapeutic Carnoy's formulations on hard human tissues: A Raman microspectroscopy, microhardness, and scanning electron microscopy study
- Creators
- Francisco Samuel Rodrigues Carvalho - Universidade Federal do CearáVictor Pinheiro Feitosa - Paulo Picanço School of DentistryPaulo Goberlânio de Barros Silva - UnichristusEduardo Costa Studart Soares - Hospital Universitário Walter CantídioThyciana Rodrigues Ribeiro - Universidade Federal do CearáCristiane Sá Roriz Fonteles - Universidade Federal do CearáFábio Wildson Gurgel Costa - Hospital Universitário Walter Cantídio
- Resource Type
- Journal article
- Publication Details
- Journal of cranio-maxillo-facial surgery, Vol.46(5), pp.749-758
- DOI
- 10.1016/j.jcms.2018.02.006
- PMID
- 29655532
- ISSN
- 1010-5182
- eISSN
- 1878-4119
- Language
- English
- Date published
- 05/01/2018
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984654469502771
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