Journal article
Evaluation of neutrophilic CD64, interleukin 10 and procalcitonin as diagnostic markers of early- and late-onset neonatal sepsis
Scandinavian journal of infectious diseases, Vol.42(4), pp.299-305
2010
DOI: 10.3109/00365540903449832
PMID: 20085423
Abstract
Abstract
The assay of infection markers can improve diagnostic sensitivity in neonatal sepsis. We determined the levels of neutrophilic CD64 (nCD64), procalcitonin (PCT) and interleukin 10 (IL-10) in infants with neonatal sepsis. Forty-nine newborn infants who met the criteria of sepsis were subjected to a routine sepsis evaluation as well as measurement of PCT and IL-10 levels and nCD64 expression. Of these 49 'infected' infants, 16 had a positive blood culture (culture-positive sepsis) and 33 infants were diagnosed to have clinical sepsis with negative blood cultures (culture-negative sepsis). Another 49 healthy newborn infants were included as a control group. The sensitivity, specificity, positive predictive value and negative predictive value of PCT, IL-10 and nCD64 for the diagnosis of sepsis were determined. IL-10 had the highest sensitivity of 92% and specificity of 84% using a cut-off of ≥17.3 pg/ml. For PCT, the highest sensitivity of 65% and specificity of 60% were found at a cut-off value of ≥36.4 pg/ml. nCD64 had a maximal sensitivity of 92% and specificity of 71% at a cut-off value of 2.6%. Combinations of different markers may improve the sensitivity and specificity of biomarker tests. We found that the best combination was IL-10 and nCD64, which together provided sensitivity of 95% and specificity of 83%, and a negative predictive value of 86%.
Details
- Title: Subtitle
- Evaluation of neutrophilic CD64, interleukin 10 and procalcitonin as diagnostic markers of early- and late-onset neonatal sepsis
- Creators
- Alaa A.H Zeitoun - Paediatric DepartmentSuzan S Gad - Paediatric DepartmentFadia M Attia - Clinical Pathology Department, Faculty of Medicine, Suez Canal University, EgyptAsmaa S Abu Maziad - Paediatric DepartmentEdward F Bell - Pediatric Department, Carver College of Medicine, University of Iowa, Iowa, USA
- Resource Type
- Journal article
- Publication Details
- Scandinavian journal of infectious diseases, Vol.42(4), pp.299-305
- Publisher
- Informa UK Ltd. (Informa Healthcare, Taylor & Francis AS)
- DOI
- 10.3109/00365540903449832
- PMID
- 20085423
- ISSN
- 0036-5548
- eISSN
- 1651-1980
- Language
- English
- Date published
- 2010
- Academic Unit
- Stead Family Department of Pediatrics; Neonatology
- Record Identifier
- 9984093492102771
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