Evaluation of pneumococcal serotyping by multiplex PCR and quellung reactions

Sandra S Richter; Kristopher P Heilmann; Cassie L Dohrn; Fathollah Riahi; Daniel J Diekema; Gary V Doern

doi:10.1128/JCM.01876-13

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Evaluation of pneumococcal serotyping by multiplex PCR and quellung reactions

Journal article

Open access

Peer reviewed

Evaluation of pneumococcal serotyping by multiplex PCR and quellung reactions

Sandra S Richter, Kristopher P Heilmann, Cassie L Dohrn, Fathollah Riahi, Daniel J Diekema and Gary V Doern

Journal of clinical microbiology, Vol.51(12), pp.4193-4195

12/2013

DOI: 10.1128/JCM.01876-13

PMCID: PMC3838036

PMID: 24025905

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Abstract

Screening of 1,750 pneumococcal isolates for common serotypes by PCR was followed by Quellung reaction analysis of PCR-negative isolates with a comparison to the conventional (Quellung reaction only) approach. PCR agreed with Quellung reaction results for 99% of isolates. The sequential PCR/Quellung reaction algorithm is accurate and more cost-effective than the conventional approach.

Multiplex Polymerase Chain Reaction - methods

Streptococcus pneumoniae - classification

Algorithms

Streptococcus pneumoniae - isolation & purification

Humans

Molecular Typing - methods

Streptococcus pneumoniae - genetics

Pneumococcal Infections - microbiology

Serotyping - methods

Streptococcus pneumoniae - immunology

Details

Title: Subtitle: Evaluation of pneumococcal serotyping by multiplex PCR and quellung reactions
Creators: Sandra S Richter - Cleveland Clinic, Cleveland, Ohio, USA
Kristopher P Heilmann
Cassie L Dohrn
Fathollah Riahi
Daniel J Diekema
Gary V Doern
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.51(12), pp.4193-4195
Publisher: United States
DOI: 10.1128/JCM.01876-13
PMID: 24025905
PMCID: PMC3838036
ISSN: 1098-660X
eISSN: 1098-660X
Language: English
Date published: 12/2013
Academic Unit: Infectious Diseases; Epidemiology; Pathology; Internal Medicine
Record Identifier: 9983986271402771

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