Journal article
Evaluation of renal metabolic response to partial ureteral obstruction with hyperpolarized C-13 MRI
NMR in biomedicine, Vol.31(1), e3846
01/01/2018
DOI: 10.1002/nbm.3846
PMCID: PMC5736002
PMID: 29130537
Abstract
Hyperpolarized C-13 magnetic resonance imaging (MRI) may be used to non-invasively image the transport and chemical conversion of C-13-labeled compounds in vivo. In this study, we utilize hyperpolarized C-13 MRI to evaluate metabolic markers in the kidneys longitudinally in a mouse model of partial unilateral ureteral obstruction (pUUO). Partial obstruction was surgically induced in the left ureter of nine adult mice, leaving the right ureter as a control. H-1 and hyperpolarized [1-C-13]pyruvate MRI of the kidneys was performed 2days prior to surgery (baseline) and at 3, 7 and 14days post-surgery. Images were evaluated for changes in renal pelvis volume, pyruvate, lactate and the lactate to pyruvate ratio. After 14days, mice were sacrificed and immunohistological staining of both kidneys for collagen fibrosis (picrosirius red) and macrophage infiltration (F4/80) was performed. Statistical analysis was performed using a linear mixed effects model. Significant kidney x time interaction effects were observed for both lactate and pyruvate, indicating that these markers changed differently between time points for the obstructed and unobstructed kidneys. Both kidneys showed an increase in the lactate to pyruvate ratio after obstruction, suggesting a shift towards glycolytic metabolism. These changes were accompanied by marked hydronephrosis, fibrosis and macrophage infiltration in the obstructed kidney, but not in the unobstructed kidney. Our results show that pUUO is associated with increased pyruvate to lactate metabolism in both kidneys, with injury and inflammation specific to the obstructed kidney. The work also demonstrates the feasibility of the use of hyperpolarized C-13 MRI to study metabolism in renal disease.
Details
- Title: Subtitle
- Evaluation of renal metabolic response to partial ureteral obstruction with hyperpolarized C-13 MRI
- Creators
- David J. Niles - University of Wisconsin–MadisonJeremy W. Gordon - University of Wisconsin–MadisonGengwen Huang - University of Wisconsin–MadisonShannon Reese - University of Wisconsin–MadisonErin B. Adamson - University of Wisconsin–MadisonArjang Djamali - University of Wisconsin–MadisonSean B. Fain - University of Wisconsin–Madison
- Resource Type
- Journal article
- Publication Details
- NMR in biomedicine, Vol.31(1), e3846
- DOI
- 10.1002/nbm.3846
- PMID
- 29130537
- PMCID
- PMC5736002
- NLM abbreviation
- NMR Biomed
- ISSN
- 0952-3480
- eISSN
- 1099-1492
- Publisher
- Wiley
- Number of pages
- 9
- Grant note
- UL1TR000427 / National Center for Advancing Translational Sciences; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Advancing Translational Sciences (NCATS) R01DK092454; R01DK073680 / NIH/NIDDK; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) T32CA009206 / NIH/NCI; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) UL1TR002373 / NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Advancing Translational Sciences (NCATS) R01DK092454 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
- Language
- English
- Date published
- 01/01/2018
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Electrical and Computer Engineering; Health, Sport, and Human Physiology
- Record Identifier
- 9984275056002771
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