Evaluation of root canal microorganisms isolated from teeth with endodontic failure and their antimicrobial susceptibility

E T Pinheiro; B P F A Gomes; C C R Ferraz; F B Teixeira; A A Zaia; F J Souza Filho

doi:10.1034/j.1399-302X.2003.00058.x

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Journal article

Evaluation of root canal microorganisms isolated from teeth with endodontic failure and their antimicrobial susceptibility

E T Pinheiro, B P F A Gomes, C C R Ferraz, F B Teixeira, A A Zaia and F J Souza Filho

Oral microbiology and immunology, Vol.18(2), pp.100-103

04/2003

DOI: 10.1034/j.1399-302X.2003.00058.x

PMID: 12654099

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Abstract

Studies of the microbiota from the canals of teeth with failure of endodontic therapy have revealed that it differs markedly from that of untreated necrotic dental pulps. This study aimed to evaluate the microbiota of 30 root-filled teeth with persisting periapical lesions and to test the antibiotic susceptibility of the most prevalent species. Microbial samples, isolation and speciation were done using advanced microbiologic techniques for anaerobic species. A total of 55 bacterial species were isolated, 80% were gram-positives and 58% facultative anaerobic microorganisms. The bacterial genera most frequently recovered were Enterococcus, Streptococcus, Peptostreptococcus and Actinomyces. Antibiotic sensitivity of Enterococcus faecalis and Peptostreptococcus spp. was accomplished with the E-test system. All species studied were susceptible to benzylpenicillin, amoxicillin, amoxicillin combined with clavulanate. However, 20% of the E.faecalis strains were resistant to erythromycin and 60% to azithromycin. It was concluded that microbial flora in canals after endodontic failure comprised predominantly facultative anaerobes and gram-positive species. E.faecalis was the species most frequently isolated and showed erythromycin and azithromycin resistance among the isolates.

Actinomyces - classification

Periapical Diseases - microbiology

Enterococcus faecalis - drug effects

Peptostreptococcus - drug effects

Root Canal Therapy

Actinomyces - drug effects

Macrolides

Humans

Drug Resistance, Bacterial

Lactams

Dental Pulp Cavity - microbiology

Drug Therapy, Combination - pharmacology

Streptococcus - drug effects

Streptococcus - classification

Bacteria, Anaerobic - classification

Treatment Failure

Bacteriological Techniques

Anti-Bacterial Agents - pharmacology

Enterococcus - drug effects

Bacteria, Anaerobic - drug effects

Penicillins - pharmacology

Enterococcus - classification

Peptostreptococcus - classification

Details

Title: Subtitle: Evaluation of root canal microorganisms isolated from teeth with endodontic failure and their antimicrobial susceptibility
Creators: E T Pinheiro - Endodontic Area, Dental School of Piracicaba, University of Campinas, Piracicaba, SP, Brazil
B P F A Gomes
C C R Ferraz
F B Teixeira
A A Zaia
F J Souza Filho
Resource Type: Journal article
Publication Details: Oral microbiology and immunology, Vol.18(2), pp.100-103
Publisher: Denmark
DOI: 10.1034/j.1399-302X.2003.00058.x
PMID: 12654099
ISSN: 0902-0055
eISSN: 1399-302X
Language: English
Date published: 04/2003
Academic Unit: Endodontics
Record Identifier: 9984065808302771

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