Evaluation of serum and ocular levels of membrane attack complex and C-reactive protein in CFH-genotyped human donors

Kathleen R Chirco; Miles J Flamme-Wiese; Jill S Wiley; Lawrence A Potempa; Edwin M Stone; Budd A Tucker; Robert F Mullins

doi:10.1038/s41433-018-0170-8

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Evaluation of serum and ocular levels of membrane attack complex and C-reactive protein in CFH-genotyped human donors

Journal article

Open access

Peer reviewed

Evaluation of serum and ocular levels of membrane attack complex and C-reactive protein in CFH-genotyped human donors

Kathleen R Chirco, Miles J Flamme-Wiese, Jill S Wiley, Lawrence A Potempa, Edwin M Stone, Budd A Tucker and Robert F Mullins

Eye (London), Vol.32(11), pp.1740-1742

11/2018

DOI: 10.1038/s41433-018-0170-8

PMCID: PMC6224589

PMID: 30013157

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Abstract

There is a considerable body of evidence demonstrating a link between the membrane attack complex (MAC) and age-related macular degeneration (AMD), and between C-reactive protein (CRP) and AMD. Both the MAC and the monomeric form of CRP (mCRP) accumulate within the choriocapillaris in AMD. However, the precise contribution of these species to AMD pathophysiology has not been fully elucidated. We sought to directly assess CRP and MAC levels between human serum and ocular tissues from the same CFH Y402H genotyped donors using ELISA of serum and RPE/choroid proteins. The Y402H polymorphism was associated with significantly increased MAC in RPE/choroid samples, but not in the serum, in a previously unstudied cohort. While MAC levels in the choroid were independent of circulating levels, choroidal CRP was correlated to serum levels. These data provide further evidence for local activation of complement within the choriocapillaris in AMD.

Biomarkers - metabolism

Retinal Pigment Epithelium - metabolism

Choroid - metabolism

Complement Membrane Attack Complex - metabolism

Humans

Genotype

Male

Macular Degeneration - metabolism

C-Reactive Protein - metabolism

Macular Degeneration - genetics

Female

Aged

Polymorphism, Single Nucleotide

Complement Factor H - genetics

Details

Title: Subtitle: Evaluation of serum and ocular levels of membrane attack complex and C-reactive protein in CFH-genotyped human donors
Creators: Kathleen R Chirco - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, IA, USA
Miles J Flamme-Wiese - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, IA, USA
Jill S Wiley - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, IA, USA
Lawrence A Potempa - Roosevelt University College of Pharmacy, Schaumburg, IL, USA
Edwin M Stone - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, IA, USA
Budd A Tucker - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, IA, USA
Robert F Mullins - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa City, IA, USA. Robert-Mullins@uiowa.edu
Resource Type: Journal article
Publication Details: Eye (London), Vol.32(11), pp.1740-1742
Publisher: England
DOI: 10.1038/s41433-018-0170-8
PMID: 30013157
PMCID: PMC6224589
ISSN: 0950-222X
eISSN: 1476-5454
Grant note: R01 EY026087 / NEI NIH HHS R01 EY024605 / NEI NIH HHS EY025580 / NEI NIH HHS
Language: English
Date published: 11/2018
Academic Unit: The University of Iowa Institute for Vision Research; Iowa Neuroscience Institute; John and Marcia Carver Nonprofit Genetic Testing Laboratory; Ophthalmology and Visual Sciences
Record Identifier: 9984070766402771

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