Journal article
Evaluation of the Ames Seralyzer for Therapeutic Drug Monitoring of Theophylline
Drug intelligence & clinical pharmacy, Vol.20(2), pp.118-121
02/1986
DOI: 10.1177/106002808602000204
PMID: 3512223
Abstract
Dry reagent technology and reflectance photometry are combined in the Ames Seralyzer to offer a solid-phase plastic strip assay methodology. Light reflected from serum placed on the antibody-impregnated strip is quantitated with a two-point standard curve to display a theophylline result in 90 seconds. The accuracy and precision of the Seralyzer for serum theophylline are compared to the enzyme-multiplied immunoassay technique (Emit) which is commonly used to determine drug concentrations. Liquid calibrators (5 and 25 μg/ml) and liquid-spiked sera (10, 15, and 20 μg/ml) were measured six times daily simultaneously by both methods for twenty days.
Eighty patient samples (theophylline range: 0–33 μg/ml) were measured twice each during the twenty days. Acceptable assay limits were established and maintained by measuring 15 μg/ml spiked sera prior to and during the evaluation period. All within-run and between-run means for the Seralyzer and Emit were within ± 1 μg/ml of the spiked value. All ranges of within-run and between-run means were within ± 2 μg/ml of the spiked value. Mean between-run coefficients of variation for the Seralyzer at the 5, 10, 15, 20, and 25 μg/ml values were 11.5, 6.7, 4.9, 4.6, and 4.9 percent, respectively. Linear regression on the 80 patient samples gave a slope of 1.02, intercept of −0.28, and correlation of 0.984. The Seralyzer's accuracy and precision compare favorably with Emit for theophylline determinations greater than 7.5 μg/ml.
Details
- Title: Subtitle
- Evaluation of the Ames Seralyzer for Therapeutic Drug Monitoring of Theophylline
- Creators
- Leigh M. VaughanMiles M. WeinbergerGary Milavetz
- Contributors
- William J. Taylor (Editor)
- Resource Type
- Journal article
- Publication Details
- Drug intelligence & clinical pharmacy, Vol.20(2), pp.118-121
- DOI
- 10.1177/106002808602000204
- PMID
- 3512223
- ISSN
- 0012-6578
- eISSN
- 1542-6270
- Language
- English
- Date published
- 02/1986
- Academic Unit
- Pharmacy; Stead Family Department of Pediatrics; Pharmacy Practice and Science
- Record Identifier
- 9984365905302771
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