Journal article
Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Nonfermenting Gram-Negative Bacilli Isolated from Cultures from Cystic Fibrosis Patients
Journal of clinical microbiology, Vol.50(6), pp.2034-2039
06/2012
DOI: 10.1128/JCM.00330-12
PMCID: PMC3372130
PMID: 22495566
Abstract
The Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry (MS) instruments were evaluated for the identification of nonfermenting Gram-negative bacilli (NFGNB) by a blinded comparison to conventional biochemical or molecular methods. Two hundred NFGNB that were recovered from cultures from cystic fibrosis patients in the University of Iowa Health Care (UIHC) Microbiology Laboratory between 1 January 2006 and 31 October 2010 were sent to Mayo Clinic for analysis with the Bruker Biotyper (software version 3.0) and to bioMérieux for testing with Vitek MS (SARAMIS database version 3.62). If two attempts at direct colony testing failed to provide an acceptable MALDI-TOF identification, an extraction procedure was performed. The MS identifications from both of these systems were provided to UIHC for comparison to the biochemical or molecular identification that had been reported in the patient record. Isolates with discordant results were analyzed by 16S rRNA gene sequencing at UIHC. After discrepancy testing, the Bruker Biotyper result agreed with the biochemical or molecular method, with 72.5% of isolates to the species level, 5.5% to the complex level, and 19% to the genus level (3% not identified). The level of agreement for Vitek MS was 80% species, 3.5% complex, 6% genus, and 3.5% family (7% not identified). Both MS systems provided rapid (≤3 min per isolate) and reliable identifications. The agreement of combined species/complex/genus-level identification with the reference method was higher for the Bruker Biotyper (97% versus 89.5%,
P
= 0.004) but required an extraction step more often. Species-level agreement with the reference method was similar for both MS systems (72.5% and 80%,
P
= 0.099).
Details
- Title: Subtitle
- Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Nonfermenting Gram-Negative Bacilli Isolated from Cultures from Cystic Fibrosis Patients
- Creators
- Daniel C Marko - Univ. of Iowa Health Care, Iowa City, Iowa, USARyan T Saffert - Mayo Clinic, Rochester, Minnesota, USAScott A Cunningham - Mayo Clinic, Rochester, Minnesota, USAJay Hyman - bioMérieux Inc., Durham, North Carolina, USAJohn Walsh - bioMérieux Inc., Durham, North Carolina, USASophie Arbefeville - Univ. of Iowa Health Care, Iowa City, Iowa, USAWanita Howard - Univ. of Iowa Health Care, Iowa City, Iowa, USAJon Pruessner - Univ. of Iowa Health Care, Iowa City, Iowa, USANedal Safwat - bioMérieux Inc., Durham, North Carolina, USAFranklin R Cockerill - Mayo Clinic, Rochester, Minnesota, USAAaron D Bossler - Univ. of Iowa Health Care, Iowa City, Iowa, USARobin Patel - Mayo Clinic, Rochester, Minnesota, USASandra S Richter - Cleveland Clinic, Cleveland, Ohio, USA
- Resource Type
- Journal article
- Publication Details
- Journal of clinical microbiology, Vol.50(6), pp.2034-2039
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- DOI
- 10.1128/JCM.00330-12
- PMID
- 22495566
- PMCID
- PMC3372130
- ISSN
- 0095-1137
- eISSN
- 1098-660X
- Language
- English
- Date published
- 06/2012
- Academic Unit
- Pathology
- Record Identifier
- 9984047730502771
Metrics
29 Record Views