Evaluation of the NCCLS M44-P disk diffusion method for determining susceptibilities of 276 clinical isolates of Cryptococcus neoformans to fluconazole

M A Pfaller; S A Messer; L Boyken; C Rice; S Tendolkar; R J Hollis; D J Diekema

doi:10.1128/JCM.42.1.380-383.2004

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Evaluation of the NCCLS M44-P disk diffusion method for determining susceptibilities of 276 clinical isolates of Cryptococcus neoformans to fluconazole

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Evaluation of the NCCLS M44-P disk diffusion method for determining susceptibilities of 276 clinical isolates of Cryptococcus neoformans to fluconazole

M A Pfaller, S A Messer, L Boyken, C Rice, S Tendolkar, R J Hollis and D J Diekema

Journal of clinical microbiology, Vol.42(1), pp.380-383

01/2004

DOI: 10.1128/JCM.42.1.380-383.2004

PMCID: PMC321716

PMID: 14715784

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Abstract

We evaluated the NCCLS M44-P fluconazole disk diffusion method in comparison with the NCCLS M27-A2 broth microdilution method for determining the susceptibility of 276 isolates of Cryptococcus neoformans. Disk diffusion testing was performed using Mueller-Hinton agar supplemented with 2% glucose and 0.5 microg of methylene blue/ml. Among the 276 isolates, 259 (93.8%) were susceptible, 16 (5.8%) were susceptible--dose dependent, and 1 (0.4%) was resistant to fluconazole as determined by the NCCLS broth microdilution method. The overall categorical agreement between the two methods was 86%, with 0% very major errors, 2% major errors, and 12% minor errors. The disk diffusion method using Mueller-Hinton agar supplemented with glucose and methylene blue appears to be a useful approach for determining the fluconazole susceptibility of C. neoformans.

Antifungal Agents - pharmacology

Fluconazole - pharmacology

Microbial Sensitivity Tests - standards

Diffusion

Microbial Sensitivity Tests - methods

Cryptococcus neoformans - drug effects

Quality Control

Details

Title: Subtitle: Evaluation of the NCCLS M44-P disk diffusion method for determining susceptibilities of 276 clinical isolates of Cryptococcus neoformans to fluconazole
Creators: M A Pfaller - Department of Pathology, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA. michael-pfaller@uiowa.edu
S A Messer
L Boyken
C Rice
S Tendolkar
R J Hollis
D J Diekema
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.42(1), pp.380-383
DOI: 10.1128/JCM.42.1.380-383.2004
PMID: 14715784
PMCID: PMC321716
NLM abbreviation: J Clin Microbiol
ISSN: 0095-1137
eISSN: 1098-660X
Publisher: United States
Language: English
Date published: 01/2004
Academic Unit: Infectious Diseases; Epidemiology; Pathology; Internal Medicine
Record Identifier: 9983986260402771

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