Evaluation of the etest method using Mueller-Hinton agar with glucose and methylene blue for determining amphotericin B MICs for 4,936 clinical isolates of Candida species

M A Pfaller; L Boyken; S A Messer; S Tendolkar; R J Hollis; D J Diekema

doi:10.1128/JCM.42.11.4977-4979.2004

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Evaluation of the etest method using Mueller-Hinton agar with glucose and methylene blue for determining amphotericin B MICs for 4,936 clinical isolates of Candida species

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Evaluation of the etest method using Mueller-Hinton agar with glucose and methylene blue for determining amphotericin B MICs for 4,936 clinical isolates of Candida species

M A Pfaller, L Boyken, S A Messer, S Tendolkar, R J Hollis and D J Diekema

Journal of clinical microbiology, Vol.42(11), pp.4977-4979

11/2004

DOI: 10.1128/JCM.42.11.4977-4979.2004

PMCID: PMC525169

PMID: 15528683

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Abstract

The performance of the Etest using Mueller-Hinton agar supplemented with glucose (2%) and methylene blue (0.5 microg/ml) (MH-GMB) for amphotericin B susceptibility testing of 4,936 isolates of Candida spp. was assessed against that of Etest using RPMI agar with 2% glucose (RPG). MICs were determined by Etest in both media for all 4,936 isolates and were read after incubation for 48 h at 35 degrees C. The Candida isolates included C. albicans (n = 2,728), C. glabrata (n = 722), C. parapsilosis (n = 666), C. tropicalis (n = 528), C. krusei (n = 143), C. lusitaniae (n = 54), C. guilliermondii (n = 39), C. pelliculosa (n = 17), C. kefyr (n = 15), C. rugosa (n = 11), C. dubliniensis (n = 5), C. zeylanoides (n = 4), C. lipolytica (n = 3), and C. famata (n = 1). The Etest results with MH-GMB correlated well with those with RPG. Overall agreement was 92.9%, and agreements for individual species were as follows: C. lusitaniae, 98.1%; C. albicans, 95.1%; C. glabrata, 94.3%; C. krusei, 91.6%; C. parapsilosis, 86.6%; and C. tropicalis, 86.4%. The Etest method using MH-GMB appears to be a useful method for determining amphotericin B susceptibilities of Candida species.

Antifungal Agents - pharmacology

Candida - growth & development

Methylene Blue - metabolism

Humans

Amphotericin B - pharmacology

Agar

Candidiasis - microbiology

Candida - isolation & purification

Candida - classification

Culture Media

Glucose - metabolism

Candida - drug effects

Microbial Sensitivity Tests - methods

Details

Title: Subtitle: Evaluation of the etest method using Mueller-Hinton agar with glucose and methylene blue for determining amphotericin B MICs for 4,936 clinical isolates of Candida species
Creators: M A Pfaller - Department of Pathology, Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa, USA. michael-pfaller@uiowa.edu
L Boyken
S A Messer
S Tendolkar
R J Hollis
D J Diekema
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.42(11), pp.4977-4979
DOI: 10.1128/JCM.42.11.4977-4979.2004
PMID: 15528683
PMCID: PMC525169
NLM abbreviation: J Clin Microbiol
ISSN: 0095-1137
eISSN: 1098-660X
Publisher: United States
Language: English
Date published: 11/2004
Academic Unit: Infectious Diseases; Epidemiology; Pathology; Internal Medicine
Record Identifier: 9983986262102771

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