Journal article
Evaluation of three methods for detecting low-level bacterial contamination in intravenous solutions
American journal of health-system pharmacy, Vol.39(8), pp.1302-1305
08/01/1982
DOI: 10.1093/ajhp/39.8.1302
PMID: 6812414
Abstract
The accuracy of three sterility-testing methods in detecting low-level contamination in deliberately contaminated intravenous solutions was studied. One-liter bags of 5% dextrose (D5W) and 0.9% sodium chloride (saline) injections were contaminated with Staphylococcus epidermidis and Pseudomonas aeruginosa: approximately 101 viable bacteria were injected into each bag. Two membrane-filtration methods (Ivex-2 and Addi-Chek) and one aliquot method [twice concentrated tryptic soy broth (2X-TSB)] were used to test each of 10 deliberately contaminated solutions for both D5W and saline; 500 ml of each liter bag was filtered or added to 2X-TSB. Incubation containers were stored at 37°C and inspected at 24, 48, and 72 hours for turbidity. There was no significant difference among the three methods in the detection of contaminated saline solutions. The Addi-Chek system was significantly less effective in detecting contamination in D5W than either of the other methods. It is concluded that the Ivex-2 system is accurate and the easiest-to-use system of the three tested.
Details
- Title: Subtitle
- Evaluation of three methods for detecting low-level bacterial contamination in intravenous solutions
- Creators
- Christopher M. MillerDolores Furtado - Department of Microbiology, School of MedicineF. Mauriece Smith - Department of Pharmacy ServicesHarold N. Godwin - Department of Pharmacy Services, and Professor and Assistant Dean of Pharmacy for Medical Center AffairsLinda C. Hogan - University of Kansas Medical CenterDonald E. Letendre - American Society of Health-System Pharmacists
- Resource Type
- Journal article
- Publication Details
- American journal of health-system pharmacy, Vol.39(8), pp.1302-1305
- DOI
- 10.1093/ajhp/39.8.1302
- PMID
- 6812414
- ISSN
- 1079-2082
- eISSN
- 1535-2900
- Language
- English
- Date published
- 08/01/1982
- Academic Unit
- Pharmacy
- Record Identifier
- 9984366021702771
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