Evidence for Transfer of CMY-2 AmpC β-Lactamase Plasmids between Escherichia coli and Salmonella Isolates from Food Animals and Humans

P. L Winokur; D. L Vonstein; L. J Hoffman; E. K Uhlenhopp; G. V Doern

doi:10.1128/AAC.45.10.2716-2722.2001

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Evidence for Transfer of CMY-2 AmpC β-Lactamase Plasmids between Escherichia coli and Salmonella Isolates from Food Animals and Humans

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Evidence for Transfer of CMY-2 AmpC β-Lactamase Plasmids between Escherichia coli and Salmonella Isolates from Food Animals and Humans

P. L Winokur, D. L Vonstein, L. J Hoffman, E. K Uhlenhopp and G. V Doern

Antimicrobial agents and chemotherapy, Vol.45(10), pp.2716-2722

10/2001

DOI: 10.1128/AAC.45.10.2716-2722.2001

PMCID: PMC90722

PMID: 11557460

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Abstract

Escherichia coli is an important pathogen that shows increasing antimicrobial resistance in isolates from both animals and humans. Our laboratory recently described Salmonellaisolates from food animals and humans that expressed an identical plasmid-mediated, AmpC-like β-lactamase, CMY-2. In the present study, 59 of 377 E. coli isolates from cattle and swine (15.6%) and 6 of 1,017 (0.6%) isolates of human E. coli from the same geographic region were resistant to both cephamycins and extended-spectrum cephalosporins. AnampC gene could be amplified with CMY-2 primers in 94.8% of animal and 33% of human isolates. Molecular epidemiological studies of chromosomal DNA revealed little clonal relatedness among the animal and human E. coli isolates harboring the CMY-2 gene. The ampC genes from 10 animal and human E. coli isolates were sequenced, and all carried an identical CMY-2 gene. Additionally, all were able to transfer a plasmid containing the CMY-2 gene to a laboratory strain of E. coli. CMY-2 plasmids demonstrated two different plasmid patterns that each showed strong similarities to previously describedSalmonella CMY-2 plasmids. Additionally, Southern blot analyses using a CMY-2 probe demonstrated conserved fragments among many of the CMY-2 plasmids identified in Salmonella andE. coli isolates from food animals and humans. These data demonstrate that common plasmids have been transferred between animal-associated Salmonella and E. coli, and identical CMY-2 genes carried by similar plasmids have been identified in humans, suggesting that the CMY-2 plasmid has undergone transfer between different bacterial species and may have been transmitted between food animals and humans.

Mechanisms of Resistance

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Title: Subtitle: Evidence for Transfer of CMY-2 AmpC β-Lactamase Plasmids between Escherichia coli and Salmonella Isolates from Food Animals and Humans
Creators: P. L Winokur - University of Iowa College of Medicine
D. L Vonstein - University of Iowa College of Medicine
L. J Hoffman - Iowa State University
E. K Uhlenhopp - Iowa State University
G. V Doern - University of Iowa College of Medicine
Resource Type: Journal article
Publication Details: Antimicrobial agents and chemotherapy, Vol.45(10), pp.2716-2722
DOI: 10.1128/AAC.45.10.2716-2722.2001
PMID: 11557460
PMCID: PMC90722
NLM abbreviation: Antimicrob Agents Chemother
ISSN: 0066-4804
eISSN: 1098-6596
Publisher: American Society for Microbiology
Language: English
Date published: 10/2001
Academic Unit: Infectious Diseases; Epidemiology; Pathology; Medicine Administration; Internal Medicine
Record Identifier: 9984094747002771

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