Journal article
Evidence for an Extracellular Zinc-Veneer in Rodent Brains from Experiments with Zn-Ionophores and ZnT3 Knockouts
ACS chemical neuroscience, Vol.3(10), pp.761-766
07/04/2012
DOI: 10.1021/cn300061z
PMCID: PMC3474262
PMID: 23077720
Abstract
Ionic zinc is found at a high concentration in some glutamatergic
vesicles of the mammalian brain. Ionic zinc is also found chelated
to macromolecules in the extracellular space, constituting what has
been called the “zinc veneer”. In this communication
we show that the zinc ionophore, pyrithione, can be used to demonstrate
the presence of the veneer. Application of pyrithione without added
ionic zinc to rodent hippocampal slices mobilizes extracellular zinc,
which can be detected intracellularly by the zinc probe FluoZin-3.
In addition, we show that ZnT3 null mice, which lack the transporter
responsible for stocking synaptic vesicles, nevertheless do have a
zinc veneer, albeit diminished compared to wild type animals. The
presence of the zinc veneer in ZnT3 null mice may account for the
absence of any marked deficit in these animals.
Details
- Title: Subtitle
- Evidence for an Extracellular Zinc-Veneer in Rodent Brains from Experiments with Zn-Ionophores and ZnT3 Knockouts
- Creators
- Irma Nydegger - Departments of Biology andSean M Rumschik - Departments of Biology andJinfu Zhao - Departments of Biology andAlan R Kay - Departments of Biology and
- Resource Type
- Journal article
- Publication Details
- ACS chemical neuroscience, Vol.3(10), pp.761-766
- DOI
- 10.1021/cn300061z
- PMID
- 23077720
- PMCID
- PMC3474262
- NLM abbreviation
- ACS Chem Neurosci
- ISSN
- 1948-7193
- eISSN
- 1948-7193
- Publisher
- American Chemical Society
- Language
- English
- Date published
- 07/04/2012
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984068337002771
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