Journal article
Evidence for endocytosis of ROMK potassium channel via clathrin-coated vesicles
American journal of physiology. Renal physiology, Vol.283(4), pp.F630-F639
10/01/2002
DOI: 10.1152/ajprenal.00378.2001
PMID: 12217853
Abstract
ROMK channels are present in the cortical collecting ducts of kidney and are responsible for K
+
secretion in this nephron segment. Recent studies suggest that endocytosis of ROMK channels is important for regulation of K
+
secretion in cortical collecting ducts. We investigated the molecular mechanisms for endocytosis of ROMK channels expressed in Xenopus laevis oocytes and cultured Madin-Darby canine kidney cells. When plasma membrane insertion of newly synthesized channel proteins was blocked by incubation with brefeldin A, ROMK currents decreased with a half-time of ∼6 h. Coexpression with the Lys44→Ala dominant-negative mutant dynamin, but not wild-type dynamin, reduced the rate of reduction of ROMK in the presence of brefeldin A. Mutation of Asn371 to Ile in the putative NPXY internalization motif of ROMK1 abolished the effect of the Lys44→Ala dynamin mutant on endocytosis of the channel. Coimmunoprecipitation study and confocal fluorescent imaging revealed that ROMK channels associated with clathrin coat proteins in Madin-Darby canine kidney cells. These results provide compelling evidence for endocytosis of ROMK channels via clathrin-coated vesicles.
Details
- Title: Subtitle
- Evidence for endocytosis of ROMK potassium channel via clathrin-coated vesicles
- Creators
- Wei-Zhong Zeng - The University of Texas Southwestern Medical CenterVictor Babich - The University of Texas Southwestern Medical CenterBernardo Ortega - The University of Texas Southwestern Medical CenterRaymond Quigley - University of SheffieldStanley J. White - University of Maryland, BaltimorePaul A. Welling - The University of Texas Southwestern Medical CenterChou-Long Huang - Departments of Medicine and
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Renal physiology, Vol.283(4), pp.F630-F639
- DOI
- 10.1152/ajprenal.00378.2001
- PMID
- 12217853
- ISSN
- 1931-857X
- eISSN
- 1522-1466
- Language
- English
- Date published
- 10/01/2002
- Academic Unit
- Nephrology; Internal Medicine
- Record Identifier
- 9984359865702771
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