Evidence for the Presence of Calsequestrin in Both Peripheral and Interior Regions of Sheep Purkinje Fibers

Annelise Jorgensen; Anne McLeod; Kevin Campbell; Gerald Denney

doi:10.1161/01.RES.55.2.267

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Evidence for the Presence of Calsequestrin in Both Peripheral and Interior Regions of Sheep Purkinje Fibers

Journal article

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Evidence for the Presence of Calsequestrin in Both Peripheral and Interior Regions of Sheep Purkinje Fibers

Annelise Jorgensen, Anne McLeod, Kevin Campbell and Gerald Denney

Circulation research, Vol.55(2), pp.267-270

08/1984

DOI: 10.1161/01.RES.55.2.267

PMID: 15244571

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Abstract

Localization of calsequestrin in sheep Purkinje fibers was determined by indirect immunofluorescence labeling of cryostat sections of sheep myocardium from the intraventricular wall. The results presented show that calsequestrin is present in discrete foci at the peripheral, as well as the interior regions of the cytoplasm. Since Purkinje fibers lack transverse tubules, the presence of calsequestrin at specific foci in the interior regions of the cytoplasm in these cells suggests that calsequestrin is localized in the lumen of peripheral junctional sarcoplasmic reticu-lum, as well as in the lumen of corbular sarcoplasmic reticulum present in the I band region of the myofibrils. Assuming that the function of calsequestrin is to sequester calcium into the lumen of the sarcoplasmic reticulum, these results imply that two structurally different regions of the sarcoplasmic reticulum function as calcium storage sites in mammalian Purkinje fibers and raises the possibility that calcium storage and/or release from these two sites might be regulated differently.

Details

Title: Subtitle: Evidence for the Presence of Calsequestrin in Both Peripheral and Interior Regions of Sheep Purkinje Fibers
Creators: Annelise Jorgensen - Department of Anatomy (Histology), University of Toronto, Toronto, Canada, and Department of Physiology and Biophysics, University of Iowa, Iowa City, Iowa
Anne McLeod
Kevin Campbell
Gerald Denney
Resource Type: Journal article
Publication Details: Circulation research, Vol.55(2), pp.267-270
DOI: 10.1161/01.RES.55.2.267
PMID: 15244571
NLM abbreviation: Circ Res
ISSN: 0009-7330
eISSN: 1524-4571
Publisher: American Heart Association, Inc
Language: English
Date published: 08/1984
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
Record Identifier: 9984020793502771

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