Evidence for the existence of a surface receptor for ferriclactoferrin and ferrictransferrin associated with the plasma membrane of the protozoan parasite Leishmania donovani

B E Britigan; T S Lewis; M L McCormick; M E Wilson

doi:10.1007/978-1-4757-9068-9_16

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Evidence for the existence of a surface receptor for ferriclactoferrin and ferrictransferrin associated with the plasma membrane of the protozoan parasite Leishmania donovani

Journal article

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Evidence for the existence of a surface receptor for ferriclactoferrin and ferrictransferrin associated with the plasma membrane of the protozoan parasite Leishmania donovani

B E Britigan, T S Lewis, M L McCormick and M E Wilson

Advances in experimental medicine and biology, Vol.443, pp.135-140

1998

DOI: 10.1007/978-1-4757-9068-9_16

PMID: 9781352

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Abstract

Previous work has demonstrated the ability of the promastigote form of the protozoan parasite Leishmania chagasi to utilize iron chelated to lactoferrin and transferrin for growth and metabolism. We have obtained evidence suggesting that the promastigote form of the parasite possesses specific binding sites for lactoferrin and transferrin. Lactoferrin binding appears to be: 1) independent of whether or not the protein contains iron; 2) not inhibited by transferrin; and 3) independent of whether the organism is in log or stationary phase of growth. Transferrin binding is: 1) markedly greater if the protein is iron loaded; 2) inhibited by the presence of lactoferrin; and 3) independent of whether the organism is in log or stationary growth phase. Preliminary ligand blot analyses are consistent with the presence of a protein or proteins which bind lactoferrin and/or transferrin. The relationship to these binding sites to those described in other protozoan species requires further investigation.

Leishmania donovani - metabolism

Lactoferrin - metabolism

Animals

Ferric Compounds - metabolism

Receptors, Cell Surface - metabolism

Transferrin - analogs & derivatives

Transferrin - metabolism

Receptors, Transferrin - metabolism

Lactoferrin - analogs & derivatives

Details

Title: Subtitle: Evidence for the existence of a surface receptor for ferriclactoferrin and ferrictransferrin associated with the plasma membrane of the protozoan parasite Leishmania donovani
Creators: B E Britigan - Research Service, VA Medical Center-Iowa City, Iowa 52246, USA
T S Lewis
M L McCormick
M E Wilson
Resource Type: Journal article
Publication Details: Advances in experimental medicine and biology, Vol.443, pp.135-140
Publisher: United States
DOI: 10.1007/978-1-4757-9068-9_16
PMID: 9781352
ISSN: 0065-2598
Grant note: AI-34954 / NIAID NIH HHS AI-32135 / NIAID NIH HHS
Language: English
Date published: 1998
Academic Unit: Microbiology and Immunology; International Programs; Epidemiology; Radiation Oncology; Internal Medicine
Record Identifier: 9984002385502771

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