Journal article
Evidence for the presence of calsequestrin in two structurally different regions of myocardial sarcoplasmic reticulum
The Journal of cell biology, Vol.98(4), pp.1597-1602
1984
DOI: 10.1083/jcb.98.4.1597
PMID: 6371026
Abstract
Localization of calsequestrin in chicken ventricular muscle cells was determined by indirect immunofluorescence and immuno-Protein A-colloidal gold labeling of cryostat and ultracryotomy sections, respectively. Calsequestrin was localized in the lumen of peripheral junctional sarcoplasmic reticulum, as well as in the lumen of membrane-bound structures present in the central region of the I-band, while being absent from the lumen of the sarcoplasmic reticulum in the A-band region of the cardiac muscle cells. Since chicken ventricular muscle cells lack transverse tubules, the presence of calsequestrin in membrane bound structures in the central region of the I-band suggests that these cells contain nonjunctional regions of sarcoplasmic reticulum that are involved in Ca2+ storage and possibly Ca2+ release. It is likely that the calsequestrin containing structures present throughout the I-band region of the muscle cells correspond to specialized regions of the free sarcoplasmic reticulum in the I-band called corbular sarcoplasmic reticulum. It will be of interest to determine whether Ca2+ storage and possibly Ca2+ release from junctional and nonjunctional regions of the sarcoplasmic reticulum in chicken ventricular muscle cells are regulated by the same or different physiological signals.
Details
- Title: Subtitle
- Evidence for the presence of calsequestrin in two structurally different regions of myocardial sarcoplasmic reticulum
- Creators
- A. O JORGENSEN - Univ. Toronto, dep. anatomy (histology), Toronto ON M5S 1A8, CanadaK. P CAMPBELL - Univ. Toronto, dep. anatomy (histology), Toronto ON M5S 1A8, Canada
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.98(4), pp.1597-1602
- Publisher
- Rockefeller University Press; New York, NY
- DOI
- 10.1083/jcb.98.4.1597
- PMID
- 6371026
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Language
- English
- Date published
- 1984
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984068381102771
Metrics
9 Record Views