Journal article
Evidence for virus-mediated oncogenesis in bladder cancers arising in solid organ transplant recipients
eLife, Vol.12, e82690
03/24/2023
DOI: 10.7554/eLife.82690
PMCID: PMC10446826
PMID: 36961501
Abstract
A small percentage of bladder cancers in the general population have been found to harbor DNA viruses. In contrast, up to 25% of tumors of solid organ transplant recipients, who are at an increased risk of developing bladder cancer and have overall poorer outcome, harbor BK polyomavirus (BKPyV). To better understand the biology of the tumors and the mechanisms of carcinogenesis from potential oncoviruses, we performed whole genome and transcriptome sequencing on bladder cancer specimens from 43 transplant patients. Nearly half of tumors from this patient population contained viral sequences. The most common were from BKPyV (N=9, 21%), JC polyomavirus (N=7, 16%), carcinogenic human papillomaviruses (N=3, 7%), and torque teno viruses (N=5, 12%). Immunohistochemistry revealed variable Large T antigen expression in BKPyV-positive tumors ranging from 100% positive staining of tumor tissue to less than 1%. In most cases of BKPyV-positive tumors, the viral genome appeared to be clonally integrated into the host chromosome consistent with microhomology-mediated end joining and coincided with focal amplifications of the tumor genome similar to other virus-mediated cancers. Significant changes in host gene expression consistent with the functions of BKPyV Large T antigen were also observed in these tumors. Lastly, we identified four mutation signatures in our cases with those attributable to APOBEC3 and SBS5 being the most abundant. Mutation signatures associated with the antiviral drug, ganciclovir, and aristolochic acid, a nephrotoxic compound found in some herbal medicines, were also observed. The results suggest multiple pathways to carcinogenesis in solid organ transplant recipients with a large fraction being virus-associated.
Details
- Title: Subtitle
- Evidence for virus-mediated oncogenesis in bladder cancers arising in solid organ transplant recipients
- Creators
- Gabriel J Starrett - Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, United StatesKelly Yu - National Cancer Institute, Rockville, United StatesYelena Golubeva - Leidos Biomedical Research Inc. (United States)Petra Lenz - Leidos Biomedical Research Inc. (United States)Mary L Piaskowski - Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, United StatesDavid Petersen - Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, United StatesMichael Dean - National Cancer Institute, Rockville, United StatesAjay Israni - University of MinnesotaBrenda Y Hernandez - University of Hawaii SystemThomas C Tucker - University of KentuckyIona Cheng - University of California, San FranciscoLou Gonsalves - Connecticut Department of Public HealthCyllene R Morris - University of California, DavisShehnaz K Hussain - Cedars-Sinai Medical CenterCharles F Lynch - University of IowaReuben S Harris - University of MinnesotaLudmila Prokunina-Olsson - National Cancer Institute, Rockville, United StatesPaul S Meltzer - Molecular Genetics Section, National Cancer Institute, Bethesda, United StatesChristopher B Buck - Lab of Cellular Oncology, National Cancer Institute, Bethesda, United StatesEric A Engels - National Cancer Institute, Rockville, United States
- Resource Type
- Journal article
- Publication Details
- eLife, Vol.12, e82690
- DOI
- 10.7554/eLife.82690
- PMID
- 36961501
- PMCID
- PMC10446826
- NLM abbreviation
- Elife
- ISSN
- 2050-084X
- eISSN
- 2050-084X
- Grant note
- Intramural Research Program / NIH HHS
- Language
- English
- Date published
- 03/24/2023
- Academic Unit
- Epidemiology
- Record Identifier
- 9984381025802771
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