Journal article
Evidence of oxidative stress following the viral infection of two lepidopteran insect cell lines
Free radical biology & medicine, Vol.31(11), pp.1448-1455
2001
DOI: 10.1016/S0891-5849(01)00728-6
PMID: 11728817
Abstract
The infection of
Spodoptera frugiperda Sf-9 (Sf-9) and
Trichoplusia ni BTI-Tn-5B1-4 (Tn-5B1-4) insect cell lines with
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) resulted in increased levels of lipid hydroperoxides and protein carbonyls. In addition, the viral infection resulted in a significant decrease in the reduced glutathione to oxidized glutathione (2GSH/GSSG) ratio. These results are all consistent with an increased level of oxidative stress as a result of the viral infection. It was also observed that the oxidative damage corresponded to reduced cell viability, i.e., the results are consistent with the premise that oxidative damage contributes to cell death. Finally, the measured intracellular activities of most of the antioxidant enzymes, specifically manganese superoxide dismutase (MnSOD), ascorbate peroxidase (APOX), and catalase (CAT, not present in Sf-9 cells), did not significantly decrease following viral infection. In contrast, the measured activity of copper-zinc superoxide dismutase (CuZnSOD) decreased in the Sf-9 and Tn-5B1-4 cells following AcMNPV infection.
Details
- Title: Subtitle
- Evidence of oxidative stress following the viral infection of two lepidopteran insect cell lines
- Creators
- Ying Wang - Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, IA, USALarry W Oberley - The Free Radical and Radiation Biology Program, The University of Iowa, Iowa City, IA, USADavid W Murhammer - Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Free radical biology & medicine, Vol.31(11), pp.1448-1455
- DOI
- 10.1016/S0891-5849(01)00728-6
- PMID
- 11728817
- NLM abbreviation
- Free Radic Biol Med
- ISSN
- 0891-5849
- eISSN
- 1873-4596
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2001
- Academic Unit
- Chemical and Biochemical Engineering
- Record Identifier
- 9984004185002771
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