Journal article
Evidence that hydrogen peroxide generated by 365-nm UVA radiation is not important in mammalian cell killing
Radiation research, Vol.123(2), pp.220-223
1990
DOI: 10.2307/3577548
PMID: 2389008
Abstract
We compared measurements of cell survival and DNA single-strand breaks (SSBs) caused by hydrogen peroxide ( H2 O2) and UVA radiation (365-nm) in both a parental and a ${\rm H}_{2}{\rm O}_{2}\text{-resistant}$ variant of the Chinese hamster ovary HA1 line derived by culturing cells in progressively higher concentrations of H2 O2. Both RNA slot blot analysis and enzyme analysis confirmed that the variant possesses high levels of both catalase activity and mRNA. The variant was completely resistant to the lethal effects of H2 O2 over the concentration range tested (up to 480 μM), whereas the parental strain showed <1% survival at this concentration. Similarly, the ${\rm H}_{2}{\rm O}_{2}\text{-resistant}$ strain exhibited far fewer ${\rm SSB}_{{\rm s}}$ after exposure to H2 O2 than the parental strain. Addition of o-phenanthroline to the parental cells during H2 O2 exposure almost completely inhibited SSB induction, evidence that these ${\rm SSB}_{{\rm s}}$ are produced via the Fenton pathway of Haber-Weiss reactions. Very little difference was found between the variant and the parent after exposure to 365-nm radiation: only a minor difference in survival kinetics and no difference is SSB induction were observed between the two cell lines. These results are consistent with a hypothesis that most lethal events caused in cells by UVA occur by pathways that do not involve the H2 O2 that is produced by sensitized reactions within the cells.
Details
- Title: Subtitle
- Evidence that hydrogen peroxide generated by 365-nm UVA radiation is not important in mammalian cell killing
- Creators
- M. J Peak - Argonne National LaboratoryC. A Jones - Argonne national lab., biological medical res. div., molecular photobiology group, Argonne IL 60439, United StatesB. A Sedita - Argonne national lab., biological medical res. div., molecular photobiology group, Argonne IL 60439, United StatesE. J Dudek - Argonne national lab., biological medical res. div., molecular photobiology group, Argonne IL 60439, United StatesD. R Spitz - Argonne national lab., biological medical res. div., molecular photobiology group, Argonne IL 60439, United StatesJ. G Peak - Argonne national lab., biological medical res. div., molecular photobiology group, Argonne IL 60439, United States
- Resource Type
- Journal article
- Publication Details
- Radiation research, Vol.123(2), pp.220-223
- Publisher
- Radiation Research Society
- DOI
- 10.2307/3577548
- PMID
- 2389008
- ISSN
- 0033-7587
- eISSN
- 1938-5404
- Language
- English
- Date published
- 1990
- Academic Unit
- Pathology; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984313083002771
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