Evidence to the role of interflavan linkages and galloylation of proanthocyanidins at sustaining long-term dentin biomodification

Berdan Aydin; Ariene A Leme-Kraus; Cristina M.P Vidal; Thaiane R Aguiar; Rasika S Phansalkar; Joo-Won Nam; James B McAlpine; Shao-Nong Chen; Guido F Pauli; Ana K Bedran-Russo

doi:10.1016/j.dental.2018.11.029

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Evidence to the role of interflavan linkages and galloylation of proanthocyanidins at sustaining long-term dentin biomodification

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Evidence to the role of interflavan linkages and galloylation of proanthocyanidins at sustaining long-term dentin biomodification

Berdan Aydin, Ariene A Leme-Kraus, Cristina M.P Vidal, Thaiane R Aguiar, Rasika S Phansalkar, Joo-Won Nam, James B McAlpine, Shao-Nong Chen, Guido F Pauli and Ana K Bedran-Russo

Dental materials, Vol.35(2), pp.328-334

02/2019

DOI: 10.1016/j.dental.2018.11.029

PMCID: PMC6346742

PMID: 30580969

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https://www.ncbi.nlm.nih.gov/pmc/articles/6346742View

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Abstract

•Plant-derived proanthocyanidins are potent dentin biomodification agents containing high structural diversity.•Unique chemical features in proanthocyanidins hold important clues to the sustained interactivity with the dentin matrix components.•This study shows galloylated PAC sources exhibit high initial dentin biomodification, however the effects are not sustainable.•The interflavan monomeric linkages appears to affect the stability, where A-type linkage favors long-term stabilities.\nThe interactivity of proanthocyanidins (PACs) with collagen modulates dentin matrix biomechanics and biostability. Herein, PAC extracts selected based on structural diversity were investigated to determine key PAC features driving sustained effects on dentin matrices over a period of 18months.\nThe chemical profiles of PAC-rich plant sources, Pinus massoniana (PM), Cinnamomum verum (CV) and Hamamelis virginiana (HV) barks, as well as Vitis vinifera (VV) seeds, were obtained by diol HPLC analysis after partitioning of the extracts between methyl acetate and water. Dentin matrices (n=15) were prepared from human molars to determine the apparent modulus of elasticity over 18months of aging. Susceptibility of the dentin matrix to degradation by endogenous and exogenous proteases was determined by presence of solubilized collagen in supernatant, and resistance to degradation by bacterial collagenase, respectively. Data were analyzed using ANOVA and Games–Howell post hoc tests (α=0.05).\nAfter 18months, dentin matrices modified by PM and CV extracts, containing only non-galloylated PACs, were highly stable mechanically (p<0.05). Dentin matrices treated with CV exhibited the lowest degradation by bacterial collagenase after 1h and 18months of aging (p<0.05), while dentin matrices treated with PM showed the least mass loss and collagen solubilization by endogenous enzymes over time (p<0.05).\nResistance against long-term degradation was observed for all experimental groups; however, the most potent and long-lasting dentin biomodification resulted from non-galloylated PACs.

Biodegradation

Dentinl

Collagen

Polyphenols

Stiffness

Proanthocyanidins

Collagen cross-linkers

Details

Title: Subtitle: Evidence to the role of interflavan linkages and galloylation of proanthocyanidins at sustaining long-term dentin biomodification
Creators: Berdan Aydin - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
Ariene A Leme-Kraus - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
Cristina M.P Vidal - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
Thaiane R Aguiar - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
Rasika S Phansalkar - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA
Joo-Won Nam - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA
James B McAlpine - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA
Shao-Nong Chen - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA
Guido F Pauli - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA
Ana K Bedran-Russo - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
Resource Type: Journal article
Publication Details: Dental materials, Vol.35(2), pp.328-334
DOI: 10.1016/j.dental.2018.11.029
PMID: 30580969
PMCID: PMC6346742
NLM abbreviation: Dent Mater
ISSN: 0109-5641
eISSN: 1879-0097
Publisher: Elsevier Inc
Language: English
Date published: 02/2019
Academic Unit: Operative Dentistry
Record Identifier: 9984066092502771

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