Journal article
Evisceration of Mouse Vitreous and Retina for Proteomic Analyses
Journal of Visualized Experiments, Vol.50, e2795
04/03/2011
DOI: 10.3791/2795
PMCID: PMC3169273
PMID: 21490583
Abstract
While the mouse retina has emerged as an important genetic model for inherited retinal disease, the mouse vitreous remains to be explored. The vitreous is a highly aqueous extracellular matrix overlying the retina where intraocular as well as extraocular proteins accumulate during disease.1-3 Abnormal interactions between vitreous and retina underlie several diseases such as retinal detachment, proliferative diabetic retinopathy, uveitis, and proliferative vitreoretinopathy.1,4 The relative mouse vitreous volume is significantly smaller than the human vitreous (Figure 1), since the mouse lens occupies nearly 75% of its eye.5 This has made biochemical studies of mouse vitreous challenging. In this video article, we present a technique to dissect and isolate the mouse vitreous from the retina, which will allow use of transgenic mouse models to more clearly define the role of this extracellular matrix in the development of vitreoretinal diseases.
Details
- Title: Subtitle
- Evisceration of Mouse Vitreous and Retina for Proteomic Analyses
- Creators
- Jessica M. Skeie - University of IowaStephen H. Tsang - Columbia UniversityVinit B. Mahajan - Stanford University
- Resource Type
- Journal article
- Publication Details
- Journal of Visualized Experiments, Vol.50, e2795
- Publisher
- MyJove Corporation
- DOI
- 10.3791/2795
- PMID
- 21490583
- PMCID
- PMC3169273
- ISSN
- 1940-087X
- eISSN
- 1940-087X
- Language
- English
- Date published
- 04/03/2011
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9984627240302771
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