Evolutionary toxicogenomics: diversification of the Cyp12d1 and Cyp12d3 genes in Drosophila species

Cynthia M McDonnell; Darrin King; Josep M Comeron; Hongmei Li; Weilin Sun; May R Berenbaum; Mary A Schuler; Barry R Pittendrigh

doi:10.1007/s00239-012-9506-3

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Evolutionary toxicogenomics: diversification of the Cyp12d1 and Cyp12d3 genes in Drosophila species

Journal article

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Peer reviewed

Evolutionary toxicogenomics: diversification of the Cyp12d1 and Cyp12d3 genes in Drosophila species

Cynthia M McDonnell, Darrin King, Josep M Comeron, Hongmei Li, Weilin Sun, May R Berenbaum, Mary A Schuler and Barry R Pittendrigh

Journal of molecular evolution, Vol.74(5-6), pp.281-296

06/2012

DOI: 10.1007/s00239-012-9506-3

PMID: 22811321

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Abstract

Gene duplication and divergence are overwhelmingly considered to be the primary mechanisms by which cytochrome P450 monooxygenases (P450s) have radiated into a large and diverse gene superfamily. To address how environmental stress drives the fixation and diversification of gene duplications, we have analyzed Cyp12d1 and Cyp12d3, a pair of duplicated genes found in the sequenced Drosophila genomes of the melanogaster group. The paralog Cyp12d3, which is not found in Drosophila melanogaster, is basal to the melanogaster group, after it split from the obscura group (ca. 50 mya), and has a significant signature of positive selection in two species (D. sechellia and D. ananassae). Examination of the Cyp12d1 region in D. melanogaster wildtype and isoline populations revealed variation both in copy number and sequence, including splice-site variations, which certainly alter gene function. Further investigations of several strains have identified three cases in which differences in the Cyp12d1 gene region are associated with the differences in transcript abundance and transcriptional responses to the environmental stresses that have not been seen for other detoxificative loci. Together, these data highlight the value of using both macro- and microevolutionary approaches in studying the duplication and divergence events associated with detoxification genes and lay important groundwork for future studies in the field of evolutionary toxicogenomics, which uses the principles of phylogenetic analysis to predict possible enzymatic functions.

Polymerase Chain Reaction

Genes, Insect - genetics

Species Specificity

Genetic Loci - genetics

Molecular Sequence Data

Phylogeny

Amino Acids - genetics

Drosophila melanogaster - genetics

Drosophila - enzymology

Genetic Variation

Time Factors

Base Sequence

Toxicogenetics

Drosophila - genetics

Gene Dosage - genetics

Stress, Physiological - genetics

Sequence Analysis, DNA

Polymorphism, Genetic

Gene Expression Regulation, Enzymologic

Sequence Homology, Amino Acid

Animals

Cytochrome P-450 Enzyme System - genetics

Drosophila melanogaster - enzymology

Environment

Drosophila Proteins - genetics

Evolution, Molecular

Details

Title: Subtitle: Evolutionary toxicogenomics: diversification of the Cyp12d1 and Cyp12d3 genes in Drosophila species
Creators: Cynthia M McDonnell - University of Illinois Urbana-Champaign
Darrin King
Josep M Comeron
Hongmei Li - University of Illinois Urbana-Champaign
Weilin Sun
May R Berenbaum
Mary A Schuler
Barry R Pittendrigh
Resource Type: Journal article
Publication Details: Journal of molecular evolution, Vol.74(5-6), pp.281-296
DOI: 10.1007/s00239-012-9506-3
PMID: 22811321
NLM abbreviation: J Mol Evol
ISSN: 0022-2844
eISSN: 1432-1432
Publisher: Germany
Language: English
Date published: 06/2012
Academic Unit: Biology
Record Identifier: 9983991962602771

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