Journal article
Ex Vivo Brain Imaging in Drosophila
Cold Spring Harbor protocols, Vol.2024(6), pdb.prot108136
06/03/2024
DOI: 10.1101/pdb.prot108136
PMID: 37197828
Abstract
Analysis of neuronal circuit function in
can be facilitated with an ex vivo imaging preparation. In this approach, the brain is isolated but intact, preserving neuronal connectivity and function. The preparation has several advantages, including stability, accessibility for pharmacological manipulations, and the ability to image over several hours. The full range of genetic approaches available in
can be readily combined with pharmacological manipulations in this preparation, and numerous genetically encoded reporters are available to image cellular events, ranging from Ca
signaling to neurotransmitter release.
Details
- Title: Subtitle
- Ex Vivo Brain Imaging in Drosophila
- Creators
- Tamara Boto - Trinity College DublinSeth M Tomchik - Iowa Neuroscience Institute, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
- Resource Type
- Journal article
- Publication Details
- Cold Spring Harbor protocols, Vol.2024(6), pdb.prot108136
- DOI
- 10.1101/pdb.prot108136
- PMID
- 37197828
- NLM abbreviation
- Cold Spring Harb Protoc
- eISSN
- 1559-6095
- Language
- English
- Electronic publication date
- 05/17/2023
- Date published
- 06/03/2024
- Academic Unit
- Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Neuroscience and Pharmacology
- Record Identifier
- 9984410799502771
Metrics
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