Exacerbation of acute pancreatitis by combined cholinergic stimulation and duct obstruction

Isaac Samuel; Ashok Chaudhary; Rory A Fisher; Raymond J Joehl

doi:10.1016/j.amjsurg.2005.07.010

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Exacerbation of acute pancreatitis by combined cholinergic stimulation and duct obstruction

Journal article

Peer reviewed

Exacerbation of acute pancreatitis by combined cholinergic stimulation and duct obstruction

Isaac Samuel, Ashok Chaudhary, Rory A Fisher and Raymond J Joehl

The American journal of surgery, Vol.190(5), pp.721-724

11/2005

DOI: 10.1016/j.amjsurg.2005.07.010

PMID: 16226947

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Abstract

The role of cholinergic pathways in the pathogenesis of bile-pancreatic duct ligation (BPDL)-induced acute pancreatitis in rats remains controversial. We hypothesized that cholinergic stimulation exacerbates acute pancreatic inflammation in the presence of duct obstruction. We studied 34 rats divided into 5 groups as follows: (1) sham operation; (2) BPDL; (3) BPDL with duodenal bile-pancreatic juice (BPJ) replacement fresh from a donor rat; (4) BPDL with BPJ replacement as in 3 above, and carbachol (CCh) 5 ug/h subcutaneously; or (5) CCh 5 ug/h subcutaneously only. Rats were killed after 6 hours. The P value was less than .05 by analysis of variance. Pancreatic morphologic changes and zymogen fraction hyperamylasemia seen with duct ligation (2 vs. 1) were ameliorated significantly by duodenal BPJ replacement (3 vs. 2), but not when exogenous CCh was administered (4 vs. 3), whereas CCh alone showed no significant changes compared with sham (5 vs. 1). Cholinergic stimulation and duct obstruction synergistically amplify acinar hyperstimulation and exacerbate acute pancreatitis.

Bile Ducts, Extrahepatic - surgery

Pancreas, Exocrine - pathology

Rats

Male

alpha-Amylases - metabolism

Cholestasis, Extrahepatic - complications

Pancreas, Exocrine - drug effects

Rats, Sprague-Dawley

Stimulation, Chemical

Pancreas, Exocrine - enzymology

Carbachol - pharmacology

Pancreatitis, Acute Necrotizing - enzymology

Animals

Ligation - adverse effects

Cholinergic Agonists - pharmacology

Pancreatitis, Acute Necrotizing - etiology

Disease Models, Animal

Pancreatitis, Acute Necrotizing - pathology

Details

Title: Subtitle: Exacerbation of acute pancreatitis by combined cholinergic stimulation and duct obstruction
Creators: Isaac Samuel - Department of Surgery, Veterans Affairs Medical Center, Iowa City, IA, USA. isaac-samuel@uiowa.edu
Ashok Chaudhary
Rory A Fisher
Raymond J Joehl
Resource Type: Journal article
Publication Details: The American journal of surgery, Vol.190(5), pp.721-724
Publisher: United States
DOI: 10.1016/j.amjsurg.2005.07.010
PMID: 16226947
ISSN: 0002-9610
eISSN: 1879-1883
Grant note: K08-DK062805 / NIDDK NIH HHS
Language: English
Date published: 11/2005
Academic Unit: Iowa Neuroscience Institute; Surgery; Neuroscience and Pharmacology; Internal Medicine
Record Identifier: 9984040496502771

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