Journal article
Exacerbation of allergic inflammation in mice exposed to diesel exhaust particles prior to viral infection
Particle and fibre toxicology, Vol.6(1), pp.22-22
08/14/2009
DOI: 10.1186/1743-8977-6-22
PMCID: PMC2739151
PMID: 19682371
Abstract
Background: Viral infections and exposure to oxidant air pollutants are two of the most important inducers of asthma exacerbation. Our previous studies have demonstrated that exposure to diesel exhaust increases the susceptibility to influenza virus infections both in epithelial cells in vitro and in mice in vivo. Therefore, we examined whether in the setting of allergic asthma, exposure to oxidant air pollutants enhances the susceptibility to respiratory virus infections, which in turn leads to increased virus-induced exacerbation of asthma. Ovalbumin-sensitized ( OVA) male C57BL/6 mice were instilled with diesel exhaust particles (DEP) or saline and 24 hours later infected with influenza A/PR/8. Animals were sacrificed 24 hours post-infection and analyzed for markers of lung injury, allergic inflammation, and pro-inflammatory cytokine production.
Results: Exposure to DEP or infection with influenza alone had no significant effects on markers of injury or allergic inflammation. However, OVA-sensitized mice that were exposed to DEP and subsequently infected with influenza showed increased levels of eosinophils in lung lavage and tissue. In addition Th2-type cytokines, such as IL-4 and IL-13, and markers of eosinophil chemotaxis, such as CCL11 and CCR3, were increased in OVA-sensitized mice exposed to DEP prior to infection with influenza. These mice also showed increased levels of IL-1 alpha, but not IL-10, RANTES, and MCP-1 in lung homogenates.
Conclusion: These data suggest that in the setting of allergic asthma, exposure to diesel exhaust could enhance virus-induced exacerbation of allergic inflammation.
Details
- Title: Subtitle
- Exacerbation of allergic inflammation in mice exposed to diesel exhaust particles prior to viral infection
- Creators
- Ilona Jaspers - University of North Carolina at Chapel HillPatricia A. Sheridan - University of North Carolina at Chapel HillWenli Zhang - University of North Carolina at Chapel HillLuisa E. Brighton - University of North Carolina at Chapel HillKelly D. Chason - University of North CarolinaXiaoyang Hua - University of North Carolina at Chapel HillStephen L. Tilley - University of North Carolina at Chapel Hill
- Resource Type
- Journal article
- Publication Details
- Particle and fibre toxicology, Vol.6(1), pp.22-22
- DOI
- 10.1186/1743-8977-6-22
- PMID
- 19682371
- PMCID
- PMC2739151
- NLM abbreviation
- Part Fibre Toxicol
- ISSN
- 1743-8977
- eISSN
- 1743-8977
- Publisher
- Springer Nature
- Number of pages
- 11
- Grant note
- CR829522 / Environmental Protection Agency; United States Environmental Protection Agency CR829522 / United States Environmental Protection Agency Center for Environmental Medicine, Asthma, and Lung Biology ES013611 / National Institute for Environmental Health Sciences (NIEHS); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS) NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 08/14/2009
- Academic Unit
- Otolaryngology
- Record Identifier
- 9984311442702771
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