Exercise training induces a cardioprotective phenotype and alterations in cardiac subsarcolemmal and intermyofibrillar mitochondrial proteins

Andreas N Kavazis; Sophie Alvarez; Erin Talbert; Youngil Lee; Scott K Powers

doi:10.1152/ajpheart.01278.2008

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Exercise training induces a cardioprotective phenotype and alterations in cardiac subsarcolemmal and intermyofibrillar mitochondrial proteins

Journal article

Open access

Peer reviewed

Exercise training induces a cardioprotective phenotype and alterations in cardiac subsarcolemmal and intermyofibrillar mitochondrial proteins

Andreas N Kavazis, Sophie Alvarez, Erin Talbert, Youngil Lee and Scott K Powers

American journal of physiology. Heart and circulatory physiology, Vol.297(1), pp.H144-152

07/2009

DOI: 10.1152/ajpheart.01278.2008

PMCID: PMC2711746

PMID: 19429812

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https://doi.org/10.1152/ajpheart.01278.2008View

Published (Version of record) Open Access

Abstract

Endurance exercise is known to provide cardioprotection against ischemia-reperfusion-induced myocardial injury, and mitochondrial adaptations may play a critical role in this protection. To investigate exercise-induced changes in mitochondrial proteins, we compared the proteome of subsarcolemmal and intermyofibrillar mitochondria isolated from the myocardium of sedentary (control) and exercise-trained Sprague-Dawley rats. To achieve this goal, we utilized isobaric tags for relative and absolute quantitation, which allows simultaneous identification and quantification of proteins between multiple samples. This approach identified a total of 222 cardiac mitochondrial proteins. Importantly, repeated bouts of endurance exercise resulted in significant alterations in 11 proteins within intermyofibrillar mitochondria (seven increased; four decreased) compared with sedentary control animals. Furthermore, exercise training resulted in significant changes in two proteins within subsarcolemmal mitochondria (one increased; one decreased) compared with sedentary control animals. Differentially expressed proteins could be classified into seven functional groups, and several novel and potentially important cardioprotective mediators were identified. We conclude that endurance exercise induces alterations in mitochondrial proteome that may contribute to cardioprotective phenotype. Importantly, based on our findings, pharmacological or other interventions could be used to develop a strategy of protecting the myocardium during an ischemic attack.

Animals

Blood Pressure - physiology

Blotting, Western

Chromatography, Liquid

Coronary Circulation - physiology

Databases, Protein

Heart Diseases - genetics

Heart Diseases - prevention & control

Heart Function Tests

Heart Rate - physiology

Male

Mitochondrial Proteins - genetics

Mitochondrial Proteins - metabolism

Monoamine Oxidase - metabolism

Myocardium - metabolism

Myofibrils - metabolism

Phenotype

Physical Conditioning, Animal - physiology

Rats

Rats, Sprague-Dawley

Sarcolemma - metabolism

Trypsin - chemistry

Details

Title: Subtitle: Exercise training induces a cardioprotective phenotype and alterations in cardiac subsarcolemmal and intermyofibrillar mitochondrial proteins
Creators: Andreas N Kavazis - University of Florida
Sophie Alvarez
Erin Talbert
Youngil Lee
Scott K Powers
Resource Type: Journal article
Publication Details: American journal of physiology. Heart and circulatory physiology, Vol.297(1), pp.H144-152
DOI: 10.1152/ajpheart.01278.2008
PMID: 19429812
PMCID: PMC2711746
NLM abbreviation: Am J Physiol Heart Circ Physiol
ISSN: 0363-6135
eISSN: 1522-1539
Grant note: R01 HL067855 / NHLBI NIH HHS R01 HL067855-01A1 / NHLBI NIH HHS
Language: English
Date published: 07/2009
Academic Unit: Fraternal Order of Eagles Diabetes Research Center; Health, Sport, and Human Physiology ; Internal Medicine
Record Identifier: 9984259653702771

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