Existence and participation of xanthine oxidase in reperfusion injury of ischemic rabbit myocardium

Lance S Terada; Jeffrey D. Rubinstein; Edward J Lesnefsky; Lawrence D Horwitz; Jonathan A Leff; John E Repine

doi:10.1152/ajpheart.1991.260.3.h805

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Existence and participation of xanthine oxidase in reperfusion injury of ischemic rabbit myocardium

Journal article

Peer reviewed

Existence and participation of xanthine oxidase in reperfusion injury of ischemic rabbit myocardium

Lance S Terada, Jeffrey D. Rubinstein, Edward J Lesnefsky, Lawrence D Horwitz, Jonathan A Leff and John E Repine

The American journal of physiology, Vol.260(3 Pt 2), pp.H805-H810

03/01/1991

DOI: 10.1152/ajpheart.1991.260.3.h805

PMID: 2000975

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Abstract

Using a highly specific assay that minimizes enzyme inactivation in vitro, we found that rabbit myocardial tissue contained low levels of xanthine oxidase (XO) and xanthine dehydrogenase (XD) activity that were effectively inhibited by pretreatment of hearts with allopurinol. In parallel, allopurinol treatment also improved ventricular developed pressure, peak systolic pressure, and coronary flow in isolated hearts subjected to 30 min of normothermic global ischemia and 30 min of reperfusion. Although function was protected by allopurinol treatment, creatine kinase (CK) release was not altered by allopurinol. Inhibition of myocardial XO with allopurinol did not increase myocardial ATP or phosphocreatine. In addition, allopurinol did not scavenge superoxide anion or hydrogen peroxide in vitro. The results support the possibility that relatively low amounts of XO activity, similar to levels reported in human myocardium, may contribute to cardiac ischemia-reperfusion injury.

Allopurinol - metabolism

Allopurinol - pharmacology

Animals

Coronary Disease - enzymology

Coronary Disease - physiopathology

Creatine Kinase - metabolism

Free Radical Scavengers

Heart - physiopathology

Hydrogen Peroxide - metabolism

Myocardial Reperfusion Injury - enzymology

Myocardium - enzymology

Oxygen - metabolism

Rabbits

Ventricular Function

Xanthine Oxidase - antagonists & inhibitors

Xanthine Oxidase - metabolism

Details

Title: Subtitle: Existence and participation of xanthine oxidase in reperfusion injury of ischemic rabbit myocardium
Creators: Lance S Terada - University of Colorado Denver
Jeffrey D. Rubinstein - University of Colorado Denver
Edward J Lesnefsky - University of Colorado Denver
Lawrence D Horwitz - University of Colorado Denver
Jonathan A Leff - University of Colorado Denver
John E Repine - University of Colorado Denver
Resource Type: Journal article
Publication Details: The American journal of physiology, Vol.260(3 Pt 2), pp.H805-H810
DOI: 10.1152/ajpheart.1991.260.3.h805
PMID: 2000975
ISSN: 0002-9513
eISSN: 2163-5773
Grant note: P50-HL-40784 / NHLBI NIH HHS K08-HL-02375 / NHLBI NIH HHS P50-HL-27353 / NHLBI NIH HHS
Language: English
Date published: 03/01/1991
Academic Unit: Cardiovascular Medicine; Internal Medicine
Record Identifier: 9984656531702771

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