Journal article
Exocyst Requirement for Endocytic Traffic Directed Toward the Apical and Basolateral Poles of Polarized MDCK Cells
Molecular biology of the cell, Vol.18(10), pp.3978-3992
10/2007
DOI: 10.1091/mbc.e07-02-0097
PMCID: PMC1995710
PMID: 17686995
Abstract
The octameric exocyst complex is associated with the junctional complex and recycling endosomes and is proposed to selectively tether cargo vesicles directed toward the basolateral surface of polarized Madin-Darby canine kidney (MDCK) cells. We observed that the exocyst subunits Sec6, Sec8, and Exo70 were localized to early endosomes, transferrin-positive common recycling endosomes, and Rab11a-positive apical recycling endosomes of polarized MDCK cells. Consistent with its localization to multiple populations of endosomes, addition of function-blocking Sec8 antibodies to streptolysin-O–permeabilized cells revealed exocyst requirements for several endocytic pathways including basolateral recycling, apical recycling, and basolateral-to-apical transcytosis. The latter was selectively dependent on interactions between the small GTPase Rab11a and Sec15A and was inhibited by expression of the C-terminus of Sec15A or down-regulation of Sec15A expression using shRNA. These results indicate that the exocyst complex may be a multipurpose regulator of endocytic traffic directed toward both poles of polarized epithelial cells and that transcytotic traffic is likely to require Rab11a-dependent recruitment and modulation of exocyst function, likely through interactions with Sec15A.
Details
- Title: Subtitle
- Exocyst Requirement for Endocytic Traffic Directed Toward the Apical and Basolateral Poles of Polarized MDCK Cells
- Creators
- Asli Oztan - Laboratory of Epithelial Cell Biology/Renal Electrolyte Division of the Department of Medicine and, Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, PA 15261Mark Silvis - Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, PA 15261Ora A Weisz - Laboratory of Epithelial Cell Biology/Renal Electrolyte Division of the Department of Medicine and, Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, PA 15261Neil A Bradbury - Department of Physiology and Biophysics, Chicago Medical School, Chicago, IL 60064Shu-Chan Hsu - Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854James R Goldenring - Department of Surgery and Cell and Developmental Biology, Vanderbilt University and the Nashville Veterans Affairs Medical Center, Nashville, TN 37212; andCharles Yeaman - Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA 52242Gerard Apodaca - Laboratory of Epithelial Cell Biology/Renal Electrolyte Division of the Department of Medicine and, Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, PA 15261
- Contributors
- Keith Mostov (Editor)
- Resource Type
- Journal article
- Publication Details
- Molecular biology of the cell, Vol.18(10), pp.3978-3992
- DOI
- 10.1091/mbc.e07-02-0097
- PMID
- 17686995
- PMCID
- PMC1995710
- NLM abbreviation
- Mol Biol Cell
- ISSN
- 1059-1524
- eISSN
- 1939-4586
- Publisher
- American Society for Cell Biology
- Language
- English
- Date published
- 10/2007
- Academic Unit
- Anatomy and Cell Biology; Internal Medicine
- Record Identifier
- 9984025308702771
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