Exon-level expression profiling of ocular tissues

Alex H Wagner; V. Nikhil Anand; Wan-Heng Wang; Jon E Chatterton; Duo Sun; Allan R Shepard; Nasreen Jacobson; Iok-Hou Pang; Adam P DeLuca; Thomas L Casavant; Todd E Scheetz; Robert F Mullins; Terry A Braun; Abbot F Clark

doi:10.1016/j.exer.2013.03.004

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Exon-level expression profiling of ocular tissues

Journal article

Open access

Peer reviewed

Exon-level expression profiling of ocular tissues

Alex H Wagner, V. Nikhil Anand, Wan-Heng Wang, Jon E Chatterton, Duo Sun, Allan R Shepard, Nasreen Jacobson, Iok-Hou Pang, Adam P DeLuca, Thomas L Casavant, …

Experimental Eye Research, Vol.111, pp.105-111

06/2013

DOI: 10.1016/j.exer.2013.03.004

PMCID: PMC3664108

PMID: 23500522

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Abstract

The normal gene expression profiles of the tissues in the eye are a valuable resource for considering genes likely to be involved with disease processes. We profiled gene expression in ten ocular tissues from human donor eyes using Affymetrix Human Exon 1.0 ST arrays. Ten different tissues were obtained from six different individuals and RNA was pooled. The tissues included: retina, optic nerve head (ONH), optic nerve (ON), ciliary body (CB), trabecular meshwork (TM), sclera, lens, cornea, choroid/retinal pigment epithelium (RPE) and iris. Expression values were compared with publically available Expressed Sequence Tag (EST) and RNA-sequencing resources. Known tissue-specific genes were examined and they demonstrated correspondence of expression with the representative ocular tissues. The estimated gene and exon level abundances are available online at the Ocular Tissue Database. •We performed exon-level expression profiling in ten normal human ocular tissues.•Expression was measured with Affymetrix Human Exon 1.0 ST arrays.•We show correlation of expression results using other expression platforms.•Expression values are available at: https://genome.uiowa.edu/otdb/.

eye

database

tissues

gene expression

expression profile

Details

Title: Subtitle: Exon-level expression profiling of ocular tissues
Creators: Alex H Wagner - Department of Biomedical Engineering, University of Iowa, Iowa City, IA 52242, USA
V. Nikhil Anand - Department of Biomedical Engineering, University of Iowa, Iowa City, IA 52242, USA
Wan-Heng Wang - Department of Biomedical Engineering, University of Iowa, Iowa City, IA 52242, USA
Jon E Chatterton - Alcon Research, Ltd., Fort Worth, TX 76134-2099, USA
Duo Sun - Alcon Research, Ltd., Fort Worth, TX 76134-2099, USA
Allan R Shepard - Alcon Research, Ltd., Fort Worth, TX 76134-2099, USA
Nasreen Jacobson - Alcon Research, Ltd., Fort Worth, TX 76134-2099, USA
Iok-Hou Pang - North Texas Eye Research Institute, UNTHSC, Fort Worth, TX 76107, USA
Adam P DeLuca - Department of Biomedical Engineering, University of Iowa, Iowa City, IA 52242, USA
Thomas L Casavant - Department of Biomedical Engineering, University of Iowa, Iowa City, IA 52242, USA
Todd E Scheetz - Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, IA 52242, USA
Robert F Mullins - Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, IA 52242, USA
Terry A Braun - Department of Biomedical Engineering, University of Iowa, Iowa City, IA 52242, USA
Abbot F Clark - North Texas Eye Research Institute, UNTHSC, Fort Worth, TX 76107, USA
Resource Type: Journal article
Publication Details: Experimental Eye Research, Vol.111, pp.105-111
DOI: 10.1016/j.exer.2013.03.004
PMID: 23500522
PMCID: PMC3664108
NLM abbreviation: Exp Eye Res
ISSN: 0014-4835
eISSN: 1096-0007
Publisher: Elsevier BV
Language: English
Date published: 06/2013
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; Center for Bioinformatics and Computational Biology; Ophthalmology and Visual Sciences
Record Identifier: 9983980058702771

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