Exopolysaccharide Sugars Contribute to Biofilm Formation by Salmonella enterica Serovar Typhimurium on HEp-2 Cells and Chicken Intestinal Epithelium

Nathan A Ledeboer; Bradley D Jones

doi:10.1128/JB.187.9.3214-3226.2005

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Exopolysaccharide Sugars Contribute to Biofilm Formation by Salmonella enterica Serovar Typhimurium on HEp-2 Cells and Chicken Intestinal Epithelium

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Exopolysaccharide Sugars Contribute to Biofilm Formation by Salmonella enterica Serovar Typhimurium on HEp-2 Cells and Chicken Intestinal Epithelium

Nathan A Ledeboer and Bradley D Jones

Journal of bacteriology, Vol.187(9), pp.3214-3226

05/2005

DOI: 10.1128/JB.187.9.3214-3226.2005

PMCID: PMC1082824

PMID: 15838049

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Abstract

Recently, we demonstrated that Salmonella enterica serovar Typhimurium can form biofilm on HEp-2 cells in a type 1 fimbria-dependent manner. Previous work on Salmonella exopolysaccharide (EPS) in biofilm indicated that the EPS composition can vary based upon the substratum on which the bacterial biofilm forms. We have investigated the role of genes important in the production of colanic acid and cellulose, common components of EPS. A mutation in the colanic acid biosynthetic gene, wcaM , was introduced into S. enterica serovar Typhimurium strain BJ2710 and was found to disrupt biofilm formation on HEp-2 cells and chicken intestinal tissue, although biofilm formation on a plastic surface was unaffected. Complementation of the wcaM mutant with the functional gene restored the biofilm phenotype observed in the parent strain. A mutation in the putative cellulose biosynthetic gene, yhjN , was found to disrupt biofilm formation on HEp-2 cells and chicken intestinal epithelium, as well as on a plastic surface. Our data indicate that Salmonella attachment to, and growth on, eukaryotic cells represent complex interactions that are facilitated by species of EPS.

Microbial Communities and Interactions

Details

Title: Subtitle: Exopolysaccharide Sugars Contribute to Biofilm Formation by Salmonella enterica Serovar Typhimurium on HEp-2 Cells and Chicken Intestinal Epithelium
Creators: Nathan A Ledeboer - Department of Microbiology, Roy J. and Lucille A. Carver School of Medicine, University of Iowa, Iowa City, Iowa 52242-1109
Bradley D Jones - Department of Microbiology, Roy J. and Lucille A. Carver School of Medicine, University of Iowa, Iowa City, Iowa 52242-1109
Resource Type: Journal article
Publication Details: Journal of bacteriology, Vol.187(9), pp.3214-3226
DOI: 10.1128/JB.187.9.3214-3226.2005
PMID: 15838049
PMCID: PMC1082824
NLM abbreviation: J Bacteriol
ISSN: 0021-9193
eISSN: 1098-5530
Publisher: American Society for Microbiology
Language: English
Date published: 05/2005
Academic Unit: Microbiology and Immunology
Record Identifier: 9984083872602771

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