Exosome-Based Cell Homing and Angiogenic Differentiation for Dental Pulp Regeneration

Venkateswaran Ganesh; Dongrim Seol; Piedad C. Gomez-Contreras; Henry L. Keen; Kyungsup Shin; James A. Martin

doi:10.3390/ijms24010466

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Exosome-Based Cell Homing and Angiogenic Differentiation for Dental Pulp Regeneration

Journal article

Open access

Peer reviewed

Exosome-Based Cell Homing and Angiogenic Differentiation for Dental Pulp Regeneration

Venkateswaran Ganesh, Dongrim Seol, Piedad C. Gomez-Contreras, Henry L. Keen, Kyungsup Shin and James A. Martin

International journal of molecular sciences, Vol.24(1), p.466

12/27/2022

DOI: 10.3390/ijms24010466

PMCID: PMC9820194

PMID: 36613910

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Abstract

Exosomes have attracted attention due to their ability to promote intercellular communication leading to enhanced cell recruitment, lineage-specific differentiation, and tissue regeneration. The object of this study was to determine the effect of exosomes on cell homing and angiogenic differentiation for pulp regeneration. Exosomes (DPSC-Exos) were isolated from rabbit dental pulp stem cells cultured under a growth (Exo-G) or angiogenic differentiation (Exo-A) condition. The characterization of exosomes was confirmed by nanoparticle tracking analysis and an antibody array. DPSC-Exos significantly promoted cell proliferation and migration when treated with 5 × 108/mL exosomes. In gene expression analysis, DPSC-Exos enhanced the expression of angiogenic markers including vascular endothelial growth factor A (VEGFA), Fms-related tyrosine kinase 1 (FLT1), and platelet and endothelial cell adhesion molecule 1 (PECAM1). Moreover, we identified key exosomal microRNAs in Exo-A for cell homing and angiogenesis. In conclusion, the exosome-based cell homing and angiogenic differentiation strategy has significant therapeutic potential for pulp regeneration.

Details

Title: Subtitle: Exosome-Based Cell Homing and Angiogenic Differentiation for Dental Pulp Regeneration
Creators: Venkateswaran Ganesh - University of Iowa
Dongrim Seol - University of Iowa
Piedad C. Gomez-Contreras - University of Iowa
Henry L. Keen - University of Iowa
Kyungsup Shin - University of Iowa
James A. Martin - University of Iowa
Resource Type: Journal article
Publication Details: International journal of molecular sciences, Vol.24(1), p.466
DOI: 10.3390/ijms24010466
PMID: 36613910
PMCID: PMC9820194
NLM abbreviation: Int J Mol Sci
ISSN: 1422-0067
eISSN: 1422-0067
Grant note: DOI: 10.13039/100000072, name: National Institute of Dental and Craniofacial Research, award: 1R03DE030515-01
Language: English
Date published: 12/27/2022
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Orthodontics; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center; Iowa Institute of Human Genetics
Record Identifier: 9984354861702771

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