Journal article
Expanding the Toolbox for Label-Free Enzyme Assays: A Dinuclear Platinum(II) Complex/DNA Ensemble with Switchable Near-IR Emission
Molecules (Basel, Switzerland), Vol.24(23), p.4390
12/01/2019
DOI: 10.3390/molecules24234390
PMCID: PMC6930566
PMID: 31805648
Abstract
Switchable luminescent bioprobes whose emission can be turned on as a function of specific enzymatic activity are emerging as important tools in chemical biology. We report a promising platform for the development of label-free and continuous enzymatic assays in high-throughput mode based on the reversible solvent-induced self-assembly of a neutral dinuclear Pt(II) complex. To demonstrate the utility of this strategy, the switchable luminescence of a dinuclear Pt(II) complex was utilized in developing an experimentally simple, fast (10 min), low cost, and label-free turn-on luminescence assay for the endonuclease enzyme DNAse I. The complex displays a near-IR (NIR) aggregation-induced emission at 785 nm in aqueous solution that is completely quenched upon binding to G-quadruplex DNA from the human c-myc oncogene. Luminescence is restored upon DNA degradation elicited by exposure to DNAse I. Correlation between near-IR luminescence intensity and DNAse I concentration in human serum samples allows for fast and label-free detection of DNAse I down to 0.002 U/mL. The Pt(II) complex/DNA assembly is also effective for identification of DNAse I inhibitors, and assays can be performed in multiwell plates compatible with high-throughput screening. The combination of sensitivity, speed, convenience, and cost render this method superior to all other reported luminescence-based DNAse I assays. The versatile response of the Pt(II) complex to DNA structures promises broad potential applications in developing real-time and label-free assays for other nucleases as well as enzymes that regulate DNA topology.
Details
- Title: Subtitle
- Expanding the Toolbox for Label-Free Enzyme Assays: A Dinuclear Platinum(II) Complex/DNA Ensemble with Switchable Near-IR Emission
- Creators
- Moustafa T Gabr - Department of Chemistry, University of Iowa, Iowa City, IA 52242, USAF Christopher Pigge - Department of Chemistry, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Molecules (Basel, Switzerland), Vol.24(23), p.4390
- DOI
- 10.3390/molecules24234390
- PMID
- 31805648
- PMCID
- PMC6930566
- NLM abbreviation
- Molecules
- ISSN
- 1420-3049
- eISSN
- 1420-3049
- Language
- English
- Date published
- 12/01/2019
- Academic Unit
- Radiology; Chemistry
- Record Identifier
- 9984216677802771
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