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Expanding the phenotypic spectrum associated with ZIC1 variants: a neurodevelopmental disorder with and without craniosynostosis
Journal article   Open access   Peer reviewed

Expanding the phenotypic spectrum associated with ZIC1 variants: a neurodevelopmental disorder with and without craniosynostosis

Laura M. Watts, Michelle S.M. Chang, Elizabeth Lewis-Orr, Isaac S. Walton, Lisa Leinhos, Rebecca S. Tooze, Yang Pei, Eduardo Calpena, J. Heather Vedovato-dos-Santos, Dora Steel, …
Genetics in medicine, 102585
04/22/2026
DOI: 10.1016/j.gim.2026.102585
PMID: 42028696
url
https://doi.org/10.1016/j.gim.2026.102585View
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Abstract

ZIC1 encodes a transcription factor with critical roles in vertebrate neural and skeletal development. Heterozygous deletions encompassing ZIC1 and ZIC4 cause Dandy-Walker malformation, whilst in the final exon heterozygous ZIC1 variants result in a distinct phenotype of craniosynostosis with variable intellectual disability via a gain-of-function mechanism. We describe the largest group of individuals harboring ZIC1 variants to date, significantly expanding the phenotypic spectrum and allowing genotype-phenotype correlation. Through international collaboration we identified 18 different heterozygous ZIC1 variants from 22 families, comprising 30 individuals. Twelve families segregated a phenotype comprising craniosynostosis with facial dysmorphism, structural brain abnormalities and developmental delay, while 10 families had a neurodevelopmental disorder alone without craniosynostosis. Variants associated with craniosynostosis were clustered in the final exon (3) and were predominantly truncating variants predicted to escape nonsense-mediated decay. Variants associated with neurodevelopmental disorder alone included missense substitutions within exons 1 and 2 predicted to disrupt the normal function of the zinc finger domain, leading to loss of ZIC1 function which was confirmed in a functional assay. This study presents evidence for a ZIC1 genotype-phenotype correlation differentiating variants that cause a neurodevelopmental phenotype with and without craniosynostosis.
Craniosynostosis gain-of-function loss-of-function neurodevelopmental disorder ZIC1

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