Journal article
Expansion of a Cell Population Expressing Stem Cell Markers in Parathyroid Glands From Patients With Hyperparathyroidism
Annals of surgery, Vol.251(1), pp.107-113
2010
DOI: 10.1097/SLA.0b013e3181b5da28
PMID: 20009751
Abstract
Objective: We sought to examine abnormal parathyroid glands for the presence of stem cells.
Summary background data: Cancer stem cells have been identified in cancers from a variety of tissues as a CD44/CD24 cell population. We hypothesize that stem cells (SC) may also be involved in the pathogenesis of benign clonal expansion characteristic of hyperparathyroidism (HPT).
Methods: Under institutional review board approval, parathyroid tissue was obtained from 20 patients with HPT and analyzed by fluorescence-activated cell sorting (FACS) for the CD44/CD24 cell population. Immunohistochemistry (IHC) with CD44 antibody was correlated with FACS results.
Results: Parathyroid tissue was obtained for FACS analysis from 25 enlarged parathyroid glands from 20 patients, 17 with primary HPT, and 3 with secondary HPT. The average percent of SC defined as CD44/CD24 population was 10.93% for enlarged parathyroid glands. IHC using CD44 antibody was performed on 27 abnormal parathyroid glands and 7 normal parathyroid gland biopsies from the same patients. Although IHC was not as sensitive as FACS, comparison of IHC and FACS results for 24 abnormal glands gave a correlation coefficient of 0.52, which was statistically significant (P = 0.01, Spearman rank). By IHC, 13 of 27 abnormal glands stained 1+ to 3+ (average, 0.93) compared with no CD44 staining in normal glands, which was statistically different (mean IHC of 0 vs. 0.93, P = 0.03, Wilcoxon).
Conclusions: These novel findings demonstrate expansion of a resident cell population that expresses SC markers in abnormal parathyroid glands from patients with HPT. Our results suggest that clonal expansion of a resident SC population occurs in the pathogenesis not only of cancer, but also in benign parathyroid tumors occurring in HPT.
Details
- Title: Subtitle
- Expansion of a Cell Population Expressing Stem Cell Markers in Parathyroid Glands From Patients With Hyperparathyroidism
- Creators
- Sandy H FANG - Department of Surgery, University of Iowa, Iowa City, IA, United StatesJulie A GUIDROZ - Department of Surgery, University of Iowa, Iowa City, IA, United StatesYunxia O'MALLEY - Department of Surgery, University of Iowa, Iowa City, IA, United StatesGeeta LAL - Department of Surgery, University of Iowa, Iowa City, IA, United StatesSonia L SUGG - Department of Surgery, University of Iowa, Iowa City, IA, United StatesJames R HOWE - Department of Surgery, University of Iowa, Iowa City, IA, United StatesChris S JENSEN - Department of Pathology, University of Iowa, Iowa City, IA, United StatesRonald J WEIGEL - Department of Surgery, University of Iowa, Iowa City, IA, United States
- Resource Type
- Journal article
- Publication Details
- Annals of surgery, Vol.251(1), pp.107-113
- Publisher
- Lippincott Williams & Wilkins; Hagerstown, MD
- DOI
- 10.1097/SLA.0b013e3181b5da28
- PMID
- 20009751
- ISSN
- 0003-4932
- eISSN
- 1528-1140
- Language
- English
- Date published
- 2010
- Academic Unit
- Molecular Physiology and Biophysics; Anatomy and Cell Biology; Stead Family Department of Pediatrics; Pathology; Surgery; Gastroenterology, Hepatology, Pancreatology, and Nutrition; Biochemistry and Molecular Biology
- Record Identifier
- 9984025295302771
Metrics
12 Record Views