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Experimental analysis of bone marrow adipose tissue and bone marrow adipocytes: An update from the bone marrow adiposity society (BMAS)
Journal article   Open access   Peer reviewed

Experimental analysis of bone marrow adipose tissue and bone marrow adipocytes: An update from the bone marrow adiposity society (BMAS)

Michaela Tencerova, Biagio Palmisano, Stéphanie Lucas, Camille Attané, Kaisa K. Ivaska, Léa Loisay, Yoshiko M. Ikushima, Drenka Trivanovic, Alessandro Corsi, Adriana Roque, …
Bone Reports, Vol.26, 101861
09/2025
DOI: 10.1016/j.bonr.2025.101861
PMCID: PMC12363472
PMID: 40837072
url
https://doi.org/10.1016/j.bonr.2025.101861View
Published (Version of record) Open Access

Abstract

Bone marrow adipose tissue (BMAT) is physiologically linked to bone and energy metabolism, endocrine regulation, hematopoiesis and cancer-related processes. A key challenge in the field is that methods for isolating BMAT or bone marrow adipocytes (BMAds) are variable because there are no widely adopted standardized protocols. To generate awareness of this challenge and to establish uniformity in experimental approaches requiring isolation, storage and characterization of BMAT and BMAds, the Biobanking Working Group of the international Bone Marrow Adiposity Society (BMAS) has previously recommended experimental standards. This paper provides an update on this effort and presents current state-of-the-art methods and technical considerations for isolation and characterization of BMAT and BMAds, including currently available high-throughput omics approaches. This review provides a reference point based on the consensus view of BMAS investigators to support studies on biomedical, biological, biochemical and biophysical questions associated with bone marrow adiposity. •Bone marrow adipose tissue (BMAT) is critical for energy metabolism•BMAT supports bone homeostasis, endocrine regulation and hematopoiesis•BMAT and bone marrow adipocytes are dynamic and diverse across bone sites and species•Standardization, rigor and transparency is required to harmonize studies on BMAS•This review provides a consensus view for standards and technical considerations

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