Experimental use of an acrolein-based primer as collagen cross-linker for dentine bonding

Tatjana Maravic; Lorenzo Breschi; Allegra Comba; Sandra Ribeiro Cunha; Valeria Angeloni; Cesare Nucci; Josimeri Hebling; David Pashley; Franklin Tay; Annalisa Mazzoni

doi:10.1016/j.jdent.2017.11.006

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Experimental use of an acrolein-based primer as collagen cross-linker for dentine bonding

Journal article

Peer reviewed

Experimental use of an acrolein-based primer as collagen cross-linker for dentine bonding

Tatjana Maravic, Lorenzo Breschi, Allegra Comba, Sandra Ribeiro Cunha, Valeria Angeloni, Cesare Nucci, Josimeri Hebling, David Pashley, Franklin Tay and Annalisa Mazzoni

Journal of dentistry, Vol.68, pp.85-90

01/2018

DOI: 10.1016/j.jdent.2017.11.006

PMID: 29158188

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Abstract

The objective of the present study was to investigate the long-term effect of 0.01% acrolein (ACR) aqueous solution, employed as an additional primer, on the mechanical durability and enzymatic activity of resin-dentine interfaces created with a simplified etch-and-rinse adhesive. Dentine surfaces were etched with 35% phosphoric acid for 15s, rinsed and blot-dried. Specimens were then assigned to: Group 1: dentine pre-treated with 0.01% ACR aqueous solution for 1min and bonded with Adper Scotchbond 1 XT (SB1XT), a 2-step etch-and-rinse adhesive; Group 2: SB1XT was applied on untreated acid-etched dentine (control). Resin composite build-ups were made using Filtek Z250. Microtensile bond strength was tested by stressing sectioned specimens to failure immediately or after 1year of storage in artificial saliva at 37°C. Zymography and in-situ zymography assays were performed for examining dentine matrix metalloproteinase (MMP) activities. The use of 0.01% ACR as conditioning primer appeared to have contributed better to preservation of bond strength over time without affecting immediate bond strength. Zymography and in-situ zymography showed reduction in MMP activities after the application of ACR. Dentine collagen cross-linking produced by an ACR-based primer increases the longevity of resin-dentine bonds by reinforcement of the adhesive interface and reduction of dentine MMP activities. Further studies are required to evaluate the potential in vivo and in vivo cytotoxicity of ACR. The acrolein-based primer is a potentially useful clinical bonding tool because it demonstrates good collagen cross-linking ability within a clinically-acceptable working time. Although a low ACR concentration was employed in the present study, the cytotoxicity of ACR should be tested prior to clinical use.

Acrolein

Collagen cross-linkers

Hybrid layer durability

Matrix metalloproteinases

Details

Title: Subtitle: Experimental use of an acrolein-based primer as collagen cross-linker for dentine bonding
Creators: Tatjana Maravic - University of Novi Sad
Lorenzo Breschi - University of Bologna
Allegra Comba - University of Bologna
Sandra Ribeiro Cunha - Universidade de São Paulo
Valeria Angeloni - Private Practice, Imperia, Italy
Cesare Nucci - University of Bologna
Josimeri Hebling - Universidade Estadual Paulista (Unesp)
David Pashley - Augusta University
Franklin Tay - Augusta University
Annalisa Mazzoni - University of Bologna
Resource Type: Journal article
Publication Details: Journal of dentistry, Vol.68, pp.85-90
DOI: 10.1016/j.jdent.2017.11.006
PMID: 29158188
NLM abbreviation: J Dent
ISSN: 0300-5712
eISSN: 1879-176X
Publisher: Elsevier Ltd
Language: English
Date published: 01/2018
Academic Unit: Operative Dentistry
Record Identifier: 9984696756002771

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